CONICET | Buscador de Institutos y Recursos Humanos

Immunoaffinity assays-capillary electrophoresis (IA-CE) is a technique whichcombines immunocapture and CE separation [1-3]. In online IA-CE, a microextractor orconcentrator is introduced near the inlet of the capillary, which contains some compoundthat specifically retains the target analyte. A large volume of sample can then be injected,and the target analytes selectively captured. Washing and clean up procedures aresubsequently integrated online to remove excess sample and nonspecifically boundinterferents. The bound analytes are then eluted and separated by some mode of CE andfinally detected by usual detection techniques. Compared to ELISA techniques, IA-CE hassignificant advantages. Mainly, it is a miniaturized technique that combines speed,automation, and low sample consumption. Moreover, the analyte concentrator at theforepart of the capillary enables to immunocapture the considered analyte from a sample.Depending on the concentration of the target analytes in the sample, volumes can be variedto obtain suitable sensitivity. Compared to microliter wells commonly used inimmunoassays, the reactive surface area versus solution volume ratio is large and thediffusion distances are reduced in capillaries and microchannels [4]. In addition, a largenumber of analyte molecules are bound within a small volume, allowing a very sensitivedetection [5, 6]. Additionally, the separation step decreases the probability of false positiveresults as an additional parameter, electrophoretic mobility, could be used for increasing theidentification of the nature of the peak. Several applications of IA-CE have so far beendescribed for peptides and/or proteins. The main difference between each approach lies inthe way to develop the immunosorbent within the capillary.

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