Analysis Based on Morphological, Protein and SRAP Markers in Pea (Pisum sativum L).

ESPÓSITO, M.A.; MILANESI, L.; MARTIN, E.A,; CRAVERO, V.P.; LÓPEZ ANIDO, F.S.; COINTRY, E.L.

International Journal of Plant Breeding

Año: 2007 vol. 1 p. 129 - 134

1752-3478

Morphological, protein and SRAP markers among fourteen pea varieties (Pisum sativum L) were studied. Data on 15 morphological traits were collected and analyzed. A total of 32 protein bands and 162 polymorphic SRAP fragments were scored. A comparison between  morphological and molecular data and morphological and protein data was carried out through a Procrustes Generalized Method. Previously we carried out a Principal Component Analysis (PCA) for morphological data and Principal Coordinates Analysis (PCoA) for the protein and SRAP data. The correlation between SRAP and morphological data was R=0.56 for 2005 and R=0.80 for 2006 showing the highest correlation between both data sets for the second year what presented unfavourable environmental conditions. Meanwhile, the correlation between protein and morphological data was R=0.56 for 2005 and R=0.63 for 2006. These results suggest that exposure to a combination of environmental stresses may increase the expression of genetic variability for productive traits. Genetic diversity is the basis for successful crop  improvement and can be estimated by different methods such as protein or molecular markers but DNA markers provide an opportunity to characterize genotypes more precisely than proteins. Molecular markers were significantly correlated with markers based on agronomic traits, suggesting that the two systems give similar estimates of genetic relations among the varieties.