Effect of Dietary Astaxanthin on Free Radical Scavenging Capacity and Nitrite Stress Tolerance of Postlarvae Shrimp, Pleoticus muelleri

DÍAZ AC; VELURTAS SM; ESPINO ML; FENUCCI JL

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

AMER CHEMICAL SOC

Lugar: Washington; Año: 2014 vol. 62 p. 12326 - 12331

0021-8561

The aim of this study was to investigate the effect of astaxanthin feed supplementation and environmental nitrite stress in postlarvae of Pleoticus muelleri (15 ± 0.004 mg initial weight) under culture conditions. Diets containing three levels of astaxanthin, 0 mg kg−1 of diet (C0), 100 mg kg−1 of diet (C100), and 300 mg kg−1 of diet (C300), were used. Postlarvae fed with each diet were exposed to different concentrations of nitrite (NO2Na) (0−200 mg L−1). The 96 h median lethal concentration (LC50) values of nitrite N were 76.3, 89.7, and 157 mg L−1 for shrimps fed to C0, C100, and C300. The scavenging properties were evaluated against the stable 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical by electron resonance spectroscopy (EPR). For allfeed treatments, the extracts exhibited strong DPPH scavenging activity; however, shrimp fed with C100 and C300 showed the greatest activity to quench DPPH (62 and 59%, respectively) with respect to C0 (43%). It can be concluded that astaxanthin acts as a protector of nitrite stress in P. muelleri.