Commercial and extracted sodium alginate analysis after purification for bone tissue engineering application

MARÍA LUZ TORRES; FERNANDEZ JUAN MANUEL; ANA M. CORTIZO; TAMARA G. OBERTI ,

Mar del Plata

Congreso; LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACION CLINICA (SAIC 2016); 2016

Alginate is a natural, water-soluble, polysaccharide obtained from brown algae with interesting features (good biocompatibility, low toxicity, ease formation of hydrogel with divalent cations) for biomedical applications. These applications required highly purified materials. However the alginate extracted from brown seaweed and commercial alginate contain a large number of impurities, such as proteins and polyphenols, which might lead to an intense host immune reaction and reduce the biocompatibility of alginate. The brown algae, Undaria pinnatifida, native from Japan, China and Korea is a powerful invasive species which was introduced worldwide in different coasts, including the one of Puerto Madryn (Argentina). Sodium alginate was obtained from this seaweed. First aim of the extraction process was to convert the insoluble calcium salt into sodium alginate. We compare sodium alginate extracted from the stem and leaf of the U. pinnatifida seaweed with the commercial salt and their purified products. Sample characterization was performed by capillary viscosity, FTIR and TGA analysis.