REGULATION OF ACYL-COA SYNTHETASE 4 (ACSL4) EXPRESSION BY TRANSCRIPTIONAL AND POST TRANSCRIPTIONAL MECHANISMS IN BREAST CANCER CELLS

YANINA BENZO; MELINA A. DATTILO; JESICA PRADA; KATIA E. HELFENBERGER; LUCÍA HERRERA; CECILIA PODEROSO

Congreso; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2018

Acyl-CoA synthetase 4 (ACSL4) is an enzyme that catalyzes acyl-CoA synthesis from long chain fatty acid, being arachidonic acid itspreferred substrate. ACSL4 levels correlate with aggressive phe-notype in breast cancer. Its expression promotes tumor aggres-siveness by increasing migration, proliferation and invasion. Theaim of this work is to describe transcriptional and post traductionalmechanisms that could regulate ACSL4 expression in breast cancercell lines. We demonstrated by ChIP and by the use of a specificinhibitor that Estrogen-related receptor alpha is involved in ACSL4transcriptional regulation. We studied the participation of proteo-somal degradation. ACSL4 protein stability was tested on MCF-7breast cancer cells with cycloheximide (CHX) treatment. Immuno-blot showed a time-dependent decrease in ACSL4 levels after CHXtreatment, significant at 2h of CHX (***p