MITOCHONDRIAL RESPIRATORY PARAMETERS AND BIOGENESIS REGULATORY FACTORS ARE INCREASED BY ANGIOTENSIN II IN H295R HUMAN ADRENOCORTICAL CELL

MATÍAS BELLUNO; KATIA HELFENBERGER; ARIANA BALCAZAR MARTÍNEZ; ALEJANDRO BOZZANO; ANA FERNANDA CASTILLO; YANINA BENZO; CECILIA PODEROSO

Mar del Plata

Congreso; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2022

Mitochondrial homeostasis is preserved by the fine coordinationbetween the generation of new mitochondria by mitochondrial biogenesis;and the removal of damaged mitochondria. Biogenesis increasesoxidative phosphorylation capacity, diminishes pathologicoxidative stress and repair mitochondrial-associated dysfunctions.This process is driven by transcription factors such as PCG-1alpha,TFAM, NRF1 and usually is accompanied by an increase in mitochondrialDNA (mtDNA). Mitochondrial normal function is essentialfor hormones signal transduction pathway, such as angiotensin II(Ang II). We have previously reported that Ang II favors mitochondrialfusion in H295R human adrenocortical cells. The effect of AngII on mitochondrial regulators genes and metabolic parameters isnot known up to date. The aim of this work is to determine the effectof Ang II on several transcription factors involved in the regulationof mitochondrial parameters in H295R cells. We observed that 100nM Ang II promoted a significant increase in NRF1 mRNA levels ina time-dependent manner, as assessed by qPCR (p