INVESTIGADORES
DALLARD Bibiana Elisabet
artículos
Título:
Effect of a biological response modifier on cellular death mechanisms at drying off
Autor/es:
DALLARD, B.E.; BARAVALLE, C.; ORTEGA, H.H.; RUFFINO, V.; HEFFEL S.; CALVINHO, L.F.
Revista:
Journal of Dairy Research
Editorial:
CAMBRIDGE UNIV PRESS
Referencias:
Año: 2008 vol. 72 p. 167 - 175
ISSN:
0022-0299
Resumen:
Agents that increase natural protective mechanisms have been proposed for prevention and
treatment of intramammary infections. The objective of this study was to describe the effects of
a single intramammary infusion of a lipopolysaccharide (LPS)-based biological response modifier
(BRM) on cellular death mechanism in uninfected and Staphylococcus aureus-infected bovine
mammary glands during involution. Three groups of 12 cows, each one including 6 Staph.
aureus-infected and 6 uninfected, were infused in two mammary quarters with BRM or placebo
and slaughtered at 7, 14 and 21 d of involution. In infected quarters, BRM treatment produced a
significant increase in percent of stained epithelial cells for the apoptosis-promoting protein Bax
at every observation period. In addition, BRM produced a significant increase of immunostained
stromal cells for Bax compared with placebo-treated quarters. BRM treatment produced an
increase in percentages of epithelial cells staining with active caspase-3 at 7 d and 14 d of
involution compared with placebo-treated quarters and a significant decrease in percentages of
terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL)-positive epithelial cells
at 7 d and 21 d of involution. In addition, BRM treatment caused an increase in percentage of
stromal cells immunostaining for active caspase-3 and TUNEL. An increase of active caspase-3
and TUNEL epithelial and stromal cell immunostaining was observed in Staph. aureus-infected
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
and slaughtered at 7, 14 and 21 d of involution. In infected quarters, BRM treatment produced a
significant increase in percent of stained epithelial cells for the apoptosis-promoting protein Bax
at every observation period. In addition, BRM produced a significant increase of immunostained
stromal cells for Bax compared with placebo-treated quarters. BRM treatment produced an
increase in percentages of epithelial cells staining with active caspase-3 at 7 d and 14 d of
involution compared with placebo-treated quarters and a significant decrease in percentages of
terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL)-positive epithelial cells
at 7 d and 21 d of involution. In addition, BRM treatment caused an increase in percentage of
stromal cells immunostaining for active caspase-3 and TUNEL. An increase of active caspase-3
and TUNEL epithelial and stromal cell immunostaining was observed in Staph. aureus-infected
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
and slaughtered at 7, 14 and 21 d of involution. In infected quarters, BRM treatment produced a
significant increase in percent of stained epithelial cells for the apoptosis-promoting protein Bax
at every observation period. In addition, BRM produced a significant increase of immunostained
stromal cells for Bax compared with placebo-treated quarters. BRM treatment produced an
increase in percentages of epithelial cells staining with active caspase-3 at 7 d and 14 d of
involution compared with placebo-treated quarters and a significant decrease in percentages of
terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL)-positive epithelial cells
at 7 d and 21 d of involution. In addition, BRM treatment caused an increase in percentage of
stromal cells immunostaining for active caspase-3 and TUNEL. An increase of active caspase-3
and TUNEL epithelial and stromal cell immunostaining was observed in Staph. aureus-infected
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
aureus-infected and 6 uninfected, were infused in two mammary quarters with BRM or placebo
and slaughtered at 7, 14 and 21 d of involution. In infected quarters, BRM treatment produced a
significant increase in percent of stained epithelial cells for the apoptosis-promoting protein Bax
at every observation period. In addition, BRM produced a significant increase of immunostained
stromal cells for Bax compared with placebo-treated quarters. BRM treatment produced an
increase in percentages of epithelial cells staining with active caspase-3 at 7 d and 14 d of
involution compared with placebo-treated quarters and a significant decrease in percentages of
terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL)-positive epithelial cells
at 7 d and 21 d of involution. In addition, BRM treatment caused an increase in percentage of
stromal cells immunostaining for active caspase-3 and TUNEL. An increase of active caspase-3
and TUNEL epithelial and stromal cell immunostaining was observed in Staph. aureus-infected
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
and slaughtered at 7, 14 and 21 d of involution. In infected quarters, BRM treatment produced a
significant increase in percent of stained epithelial cells for the apoptosis-promoting protein Bax
at every observation period. In addition, BRM produced a significant increase of immunostained
stromal cells for Bax compared with placebo-treated quarters. BRM treatment produced an
increase in percentages of epithelial cells staining with active caspase-3 at 7 d and 14 d of
involution compared with placebo-treated quarters and a significant decrease in percentages of
terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL)-positive epithelial cells
at 7 d and 21 d of involution. In addition, BRM treatment caused an increase in percentage of
stromal cells immunostaining for active caspase-3 and TUNEL. An increase of active caspase-3
and TUNEL epithelial and stromal cell immunostaining was observed in Staph. aureus-infected
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
and slaughtered at 7, 14 and 21 d of involution. In infected quarters, BRM treatment produced a
significant increase in percent of stained epithelial cells for the apoptosis-promoting protein Bax
at every observation period. In addition, BRM produced a significant increase of immunostained
stromal cells for Bax compared with placebo-treated quarters. BRM treatment produced an
increase in percentages of epithelial cells staining with active caspase-3 at 7 d and 14 d of
involution compared with placebo-treated quarters and a significant decrease in percentages of
terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL)-positive epithelial cells
at 7 d and 21 d of involution. In addition, BRM treatment caused an increase in percentage of
stromal cells immunostaining for active caspase-3 and TUNEL. An increase of active caspase-3
and TUNEL epithelial and stromal cell immunostaining was observed in Staph. aureus-infected
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell
proliferation during mammary involution.
aureus-infected and 6 uninfected, were infused in two mammary quarters with BRM or placebo
and slaughtered at 7, 14 and 21 d of involution. In infected quarters, BRM treatment produced a
significant increase in percent of stained epithelial cells for the apoptosis-promoting protein Bax
at every observation period. In addition, BRM produced a significant increase of immunostained
stromal cells for Bax compared with placebo-treated quarters. BRM treatment produced an
increase in percentages of epithelial cells staining with active caspase-3 at 7 d and 14 d of
involution compared with placebo-treated quarters and a significant decrease in percentages of
terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL)-positive epithelial cells
at 7 d and 21 d of involution. In addition, BRM treatment caused an increase in percentage of
stromal cells immunostaining for active caspase-3 and TUNEL. An increase of active caspase-3
and TUNEL epithelial and stromal cell immunostaining was observed in Staph. aureus-infected
compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining,
was increased in epithelial and stromal cells from Staph. aureus-infected compared with
uninfected quarters at every observation period. These results provide new insights into the
mechanism of mammary cell death in uninfected and