Poster - ?Monothiol Grxs: small proteins involved in many cellular processes in Trypanosoma cruzi?

SASONI, N; FARAL-TELLO, P; PIÑEYRO, MD; PARODI-TALICE, A; GUERRERO, SA; ROBELLO, C; IGLESIAS, AA; ARIAS, DG

Paraná

Congreso; SAIB LIV Reunión Anual; 2018

SAIB

Glutaredoxins (Grx) are small proteins linked toredox metabolism and intracellular iron homeostasis. Trypanosoma cruzi is the protozoan parasite and causal agent ofChagas Disease. In this work, the functional properties of T. cruzi monotiolic Grxs are presented. The recombinant proteinswere able to catalyze the in vitroreduction of GSSG using T(SH)2 as an electron donor. The recombinantproteins were able to coordinate iron-sulfur cluster (ISC) by an in vitro reconstitution assay.Absorption spectra revealed two characteristic peaks of Grx-ISC complexes. Inaddition, gel filtration chromatography profiles indicated that the Grx-ISCcomplexs is formed by Grx dimeric species. On the other hand, yeast complementationexperiments (with grx mutants)demonstrated that the phenotype of the Dgrx5 and Dgrx4Dgrx5 mutants could be rescued by complementationwith the heterologous Grxs, partially suppressing the sensitivity of the cellsto exogenous oxidants. In parallel, T.cruzi Grxs overexpressing epimastigote cells showed increased oxidativestress tolerance and raise the mammalian cells infection capacity (ofrecombinant trypomastigotes). These results indicate that T. cruzi Grxs could participates in processes related to theinfective capacity of this parasite. These results suggest that T. cruzi Grxs have important functionsin the redox metabolism and biogenesis of ISC in the parasite.