INVESTIGADORES
ARIAS Diego Gustavo
congresos y reuniones científicas
Título:
Characterization of thioredoxin system from Leptospira interrogans
Autor/es:
SASONI, NATALIA; REINOSO, ANAHÍ; ARIAS, DIEGO G.; IGLESIAS, ALBERTO A.; GUERRERO, SERGIO A.
Lugar:
Mendoza
Reunión:
Encuentro; SAIB XLVIII Reunión Anual; 2012
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Resumen:
During tissue invasion, (the causative agent of leptospirosis) is exposed to elevated amounts of exogenous reactive oxygen and nitrogen species, which are highly toxic for the bacteria. However, this pathogen is able to establish a persistent infection in its human host. The metabolic pathways for redox homeostasis in this organism are poorly understood. In this work, we present the cloning, recombinant expression and functional characterization of the thioredoxin system (thioredoxin, TRX and thioredoxin reductase, TRXR) from . The TRXR (a low molecular massTRXR) was evaluated in its ability to catalyse the NADPH dependent reduction of DTNB and TRX. TRXsystem could be assayed as a functional redox pair that, together with 2Cys typical peroxiredoxin ( 2CysPrx) mediates the NADPH-dependent reduction of hydroperoxides. Moreover, 2CysPrx was able to accept electrons from heterologous thioredoxin system and it was shown to be sensitive to overoxidation by peroxide substrate. This work strongly supports the occurrence ofTRX system in , adding value to the genome project information of this pathogen.