INVESTIGADORES
MARINA Maria
congresos y reuniones científicas
Título:
Use of transformed tobacco plants with endogenously altered polyamine pools, for studying the regulation of arbuscular mycorrhizal infection
Autor/es:
MONTES, M; MARINA, M; CALVO, C; MENENDEZ, AB; RUIZ, OA
Lugar:
Pinamar, Buenos Aires, Argentina
Reunión:
Congreso; XLI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecula; 2005
Institución organizadora:
SAIB
Resumen:
PL-P1.
USE OF TRANSFORMED TOBACCO PLANTS WITH
ENDOGENOUSLY ALTERED POLYAMINE POOLS, FOR
STUDYING THE REGULATION OF ARBUSCULAR
MYCORRHIZAL INFECTION
USE OF TRANSFORMED TOBACCO PLANTS WITH
ENDOGENOUSLY ALTERED POLYAMINE POOLS, FOR
STUDYING THE REGULATION OF ARBUSCULAR
MYCORRHIZAL INFECTION
USE OF TRANSFORMED TOBACCO PLANTS WITH
ENDOGENOUSLY ALTERED POLYAMINE POOLS, FOR
STUDYING THE REGULATION OF ARBUSCULAR
MYCORRHIZAL INFECTION
USE OF TRANSFORMED TOBACCO PLANTS WITH
ENDOGENOUSLY ALTERED POLYAMINE POOLS, FOR
STUDYING THE REGULATION OF ARBUSCULAR
MYCORRHIZAL INFECTION
USE OF TRANSFORMED TOBACCO PLANTS WITH
ENDOGENOUSLY ALTERED POLYAMINE POOLS, FOR
STUDYING THE REGULATION OF ARBUSCULAR
MYCORRHIZAL INFECTION
USE OF TRANSFORMED TOBACCO PLANTS WITH
ENDOGENOUSLY ALTERED POLYAMINE POOLS, FOR
STUDYING THE REGULATION OF ARBUSCULAR
MYCORRHIZAL INFECTION
USE OF TRANSFORMED TOBACCO PLANTS WITH
ENDOGENOUSLY ALTERED POLYAMINE POOLS, FOR
STUDYING THE REGULATION OF ARBUSCULAR
MYCORRHIZAL INFECTION
USE OF TRANSFORMED TOBACCO PLANTS WITH
ENDOGENOUSLY ALTERED POLYAMINE POOLS, FOR
STUDYING THE REGULATION OF ARBUSCULAR
MYCORRHIZAL INFECTION
USE OF TRANSFORMED TOBACCO PLANTS WITH
ENDOGENOUSLY ALTERED POLYAMINE POOLS, FOR
STUDYING THE REGULATION OF ARBUSCULAR
MYCORRHIZAL INFECTION
USE OF TRANSFORMED TOBACCO PLANTS WITH
ENDOGENOUSLY ALTERED POLYAMINE POOLS, FOR
STUDYING THE REGULATION OF ARBUSCULAR
MYCORRHIZAL INFECTION
USE OF TRANSFORMED TOBACCO PLANTS WITH
ENDOGENOUSLY ALTERED POLYAMINE POOLS, FOR
STUDYING THE REGULATION OF ARBUSCULAR
MYCORRHIZAL INFECTION
USE OF TRANSFORMED TOBACCO PLANTS WITH
ENDOGENOUSLY ALTERED POLYAMINE POOLS, FOR
STUDYING THE REGULATION OF ARBUSCULAR
MYCORRHIZAL INFECTION
USE OF TRANSFORMED TOBACCO PLANTS WITH
ENDOGENOUSLY ALTERED POLYAMINE POOLS, FOR
STUDYING THE REGULATION OF ARBUSCULAR
MYCORRHIZAL INFECTION
USE OF TRANSFORMED TOBACCO PLANTS WITH
ENDOGENOUSLY ALTERED POLYAMINE POOLS, FOR
STUDYING THE REGULATION OF ARBUSCULAR
MYCORRHIZAL INFECTION
Montes M, Marina M, Calvo C, Menéndez A, Ruiz O.
DBBE, FCEN, UBA; IIB-INTECH. Piso 4, Pab.II, Ciudad
Universitaria (1428), Cap. Fed. E-mail: ruiz@intech.gov.ar
A number of regulatory mechanisms of plant defence response
have been described during the establishment of the arbuscular
mycorrhizal (AM) In this work, we tested an experimental design
including the use of transformed tobacco plants with endogenously
altered polyamine pools, for studying the putative role of these
polycations on the regulation of AM infection. Transgenic tobacco
plants containing the oat arginine decarboxylase (ADC) gene under
the control of a tetracycline-inducible promoter, mycorrhized or
not with Glomus intraradices were used. Tetracycline induction
decreased free spermine levels in non-mycorrhized plants
transformed with the ADC gene, and increased putrescine pools in
mycorrhized plants transformed with the ADC gene. There were
no differences in mycorrhization parameters between control plants
induced or not with tetracycline, whereas transformed plants,
showed slightly, higher levels of mycorrhizal root length, entry
points and vesicles due to tetracycline induction. However the
absence of any significant correlation between shoot polyamine
levels and mycorrhizal parameters suggests that these levels may
be not directly involved in the regulation of fungal growth within
the roots. Results are discussed regarding the improvement of
experimental design.
decreased free spermine levels in non-mycorrhized plants
transformed with the ADC gene, and increased putrescine pools in
mycorrhized plants transformed with the ADC gene. There were
no differences in mycorrhization parameters between control plants
induced or not with tetracycline, whereas transformed plants,
showed slightly, higher levels of mycorrhizal root length, entry
points and vesicles due to tetracycline induction. However the
absence of any significant correlation between shoot polyamine
levels and mycorrhizal parameters suggests that these levels may
be not directly involved in the regulation of fungal growth within
the roots. Results are discussed regarding the improvement of
experimental design.
decreased free spermine levels in non-mycorrhized plants
transformed with the ADC gene, and increased putrescine pools in
mycorrhized plants transformed with the ADC gene. There were
no differences in mycorrhization parameters between control plants
induced or not with tetracycline, whereas transformed plants,
showed slightly, higher levels of mycorrhizal root length, entry
points and vesicles due to tetracycline induction. However the
absence of any significant correlation between shoot polyamine
levels and mycorrhizal parameters suggests that these levels may
be not directly involved in the regulation of fungal growth within
the roots. Results are discussed regarding the improvement of
experimental design.
decreased free spermine levels in non-mycorrhized plants
transformed with the ADC gene, and increased putrescine pools in
mycorrhized plants transformed with the ADC gene. There were
no differences in mycorrhization parameters between control plants
induced or not with tetracycline, whereas transformed plants,
showed slightly, higher levels of mycorrhizal root length, entry
points and vesicles due to tetracycline induction. However the
absence of any significant correlation between shoot polyamine
levels and mycorrhizal parameters suggests that these levels may
be not directly involved in the regulation of fungal growth within
the roots. Results are discussed regarding the improvement of
experimental design.
decreased free spermine levels in non-mycorrhized plants
transformed with the ADC gene, and increased putrescine pools in
mycorrhized plants transformed with the ADC gene. There were
no differences in mycorrhization parameters between control plants
induced or not with tetracycline, whereas transformed plants,
showed slightly, higher levels of mycorrhizal root length, entry
points and vesicles due to tetracycline induction. However the
absence of any significant correlation between shoot polyamine
levels and mycorrhizal parameters suggests that these levels may
be not directly involved in the regulation of fungal growth within
the roots. Results are discussed regarding the improvement of
experimental design.
decreased free spermine levels in non-mycorrhized plants
transformed with the ADC gene, and increased putrescine pools in
mycorrhized plants transformed with the ADC gene. There were
no differences in mycorrhization parameters between control plants
induced or not with tetracycline, whereas transformed plants,
showed slightly, higher levels of mycorrhizal root length, entry
points and vesicles due to tetracycline induction. However the
absence of any significant correlation between shoot polyamine
levels and mycorrhizal parameters suggests that these levels may
be not directly involved in the regulation of fungal growth within
the roots. Results are discussed regarding the improvement of
experimental design.
decreased free spermine levels in non-mycorrhized plants
transformed with the ADC gene, and increased putrescine pools in
mycorrhized plants transformed with the ADC gene. There were
no differences in mycorrhization parameters between control plants
induced or not with tetracycline, whereas transformed plants,
showed slightly, higher levels of mycorrhizal root length, entry
points and vesicles due to tetracycline induction. However the
absence of any significant correlation between shoot polyamine
levels and mycorrhizal parameters suggests that these levels may
be not directly involved in the regulation of fungal growth within
the roots. Results are discussed regarding the improvement of
experimental design.
decreased free spermine levels in non-mycorrhized plants
transformed with the ADC gene, and increased putrescine pools in
mycorrhized plants transformed with the ADC gene. There were
no differences in mycorrhization parameters between control plants
induced or not with tetracycline, whereas transformed plants,
showed slightly, higher levels of mycorrhizal root length, entry
points and vesicles due to tetracycline induction. However the
absence of any significant correlation between shoot polyamine
levels and mycorrhizal parameters suggests that these levels may
be not directly involved in the regulation of fungal growth within
the roots. Results are discussed regarding the improvement of
experimental design.
decreased free spermine levels in non-mycorrhized plants
transformed with the ADC gene, and increased putrescine pools in
mycorrhized plants transformed with the ADC gene. There were
no differences in mycorrhization parameters between control plants
induced or not with tetracycline, whereas transformed plants,
showed slightly, higher levels of mycorrhizal root length, entry
points and vesicles due to tetracycline induction. However the
absence of any significant correlation between shoot polyamine
levels and mycorrhizal parameters suggests that these levels may
be not directly involved in the regulation of fungal growth within
the roots. Results are discussed regarding the improvement of
experimental design.
decreased free spermine levels in non-mycorrhized plants
transformed with the ADC gene, and increased putrescine pools in
mycorrhized plants transformed with the ADC gene. There were
no differences in mycorrhization parameters between control plants
induced or not with tetracycline, whereas transformed plants,
showed slightly, higher levels of mycorrhizal root length, entry
points and vesicles due to tetracycline induction. However the
absence of any significant correlation between shoot polyamine
levels and mycorrhizal parameters suggests that these levels may
be not directly involved in the regulation of fungal growth within
the roots. Results are discussed regarding the improvement of
experimental design.
decreased free spermine levels in non-mycorrhized plants
transformed with the ADC gene, and increased putrescine pools in
mycorrhized plants transformed with the ADC gene. There were
no differences in mycorrhization parameters between control plants
induced or not with tetracycline, whereas transformed plants,
showed slightly, higher levels of mycorrhizal root length, entry
points and vesicles due to tetracycline induction. However the
absence of any significant correlation between shoot polyamine
levels and mycorrhizal parameters suggests that these levels may
be not directly involved in the regulation of fungal growth within
the roots. Results are discussed regarding the improvement of
experimental design.
decreased free spermine levels in non-mycorrhized plants
transformed with the ADC gene, and increased putrescine pools in
mycorrhized plants transformed with the ADC gene. There were
no differences in mycorrhization parameters between control plants
induced or not with tetracycline, whereas transformed plants,
showed slightly, higher levels of mycorrhizal root length, entry
points and vesicles due to tetracycline induction. However the
absence of any significant correlation between shoot polyamine
levels and mycorrhizal parameters suggests that these levels may
be not directly involved in the regulation of fungal growth within
the roots. Results are discussed regarding the improvement of
experimental design.
decreased free spermine levels in non-mycorrhized plants
transformed with the ADC gene, and increased putrescine pools in
mycorrhized plants transformed with the ADC gene. There were
no differences in mycorrhization parameters between control plants
induced or not with tetracycline, whereas transformed plants,
showed slightly, higher levels of mycorrhizal root length, entry
points and vesicles due to tetracycline induction. However the
absence of any significant correlation between shoot polyamine
levels and mycorrhizal parameters suggests that these levels may
be not directly involved in the regulation of fungal growth within
the roots. Results are discussed regarding the improvement of
experimental design.
Glomus intraradices were used. Tetracycline induction
decreased free spermine levels in non-mycorrhized plants
transformed with the ADC gene, and increased putrescine pools in
mycorrhized plants transformed with the ADC gene. There were
no differences in mycorrhization parameters between control plants
induced or not with tetracycline, whereas transformed plants,
showed slightly, higher levels of mycorrhizal root length, entry
points and vesicles due to tetracycline induction. However the
absence of any significant correlation between shoot polyamine
levels and mycorrhizal parameters suggests that these levels may
be not directly involved in the regulation of fungal growth within
the roots. Results are discussed regarding the improvement of
experimental design.