Modulation of specific Th22 response by MDR M. tuberculosis isolated in Argentina

BELEN IMPERIALE; FERREYRA MALENA; GARCIA ANA; GRAMBLICKA GEORGINA; MATTEO MARIO; MORACHO LUCILA; GONZALEZ MONTANER PABLO; PALMERO DOMINGO; MORCILLO NORA; SASIAIN MC; DE LA BARRERA SILVIA

Mar del Plata

Congreso; Congreso SAIC SAI SAFIS 2018; 2018

SAI SAIC SAFIS

Tuberculosis (TB) is one of the main infectious disease affecting humans. Innate and adaptive immune responses are needed to control M. tuberculosis (Mtb) infection. Although TH1 T lymphocytes (LT) are essential to control Mtb growth, other LT, such as TH22 would participate in this response. Previously we demonstrated that the local multidrug-resistant (MDR) Mtb strains M and Ra differentially modulated TH1 and TH17 responses. So, the aim of this study was to explore the TH22 response induced by these strains.Peripheral blood from 25 MDR-TB, 24 fully drug-susceptible (S-TB) patients and from healthy donors (HD) were evaluated. Epidemiological and bacteriological data of patients were collected. PBMCs were cultured alone or with M, Ra or H37Rv γ irradiated strains (2:1 Mtb to PBMC ratio) for 6 days. Then, cells were tested for CD4, CD8, IL-17 and IL-22 expression by FACS and IL-22 amounts were determined in cultured-supernatants by ELISA. IL-22 secretion from Mtb-stimulated PBMC was higher in MDR-TB and S-TB patients than HD (p