In vivo drug resistance mutations dynamics from early to chronic stage of infection in antiretroviral therapy-naïve HIV-infected men who have sex with men

CEVALLOS CINTIA; CULASSO ANDRÉS; URQUIZA JAVIER; OJEDA DIEGO; SUED OMAR; FIGUEROA MARIA; AVILA MARIA; DELPINO M. VICTORIA; QUARLERI JORGE

ARCHIVES OF VIROLOGY

SPRINGER WIEN

Lugar: Viena; Año: 2020

0304-8608

Human immunodeficiency virus type-1 (HIV) primary drug resistance mutations (DRM) has influenced the long-term therapeutic effects of antiretroviral treatment (ART). Drug-resistance genotyping testing based on polymerase-gene sequences obtained by next-generation sequencing (NGS) was assessed among ten ART-naïve HIV-infected men-who-have-sex-with-men (MSM; P1-P10), from acute/early to chronic stage of infection. Three out of 10 cases exhibited the presence of major (abundance ≥20%) viral populations carrying DRM at early/acute stage that later, at chronic stage, dropped drasticall (V106M) or remained highly abundant (E138A). Four individuals exhibited additional DRM (M46I/L; I47A; I54M, L100V) as HIV minority populations (abundance 2-20%) that emerged during the chronic stage but ephemerally.