B. abortus-infected B cells induce osteoclastogenesis

PESCE VIGLIETTI AYELÉN; ARRIOLA BENITEZ PAULA C; GIAMBARTOLOMEI GUILLERMO H.; DELPINO M. VICTORIA

MICROBES AND INFECTION

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2016

1286-4579

Brucellaabortus is an intracellular bacterium thatestablishes lifelong infections in livestock and humans although the mechanismsof its chronicity are poorly understood. Activated B cells have long lifespanand B. abortus infection activates Bcells. Our results indicate that the direct infection of B cells with B. abortus induced matrixmetalloproteinase-9 (MMP-9), receptor activator for NF kB ligand (RANKL), tumor necrosis factor (TNF)-α and interleukin (IL)-6secretion. In addition, supernatants from B.abortus-infected B cells induced bone marrow-derived monocytes to undergoosteoclastogenesis. Using osteoprotegerin, RANKL`s decoy receptor, wedetermined that RANKL is involved in osteoclastogenesis induced by supernatantsfrom B: abortus-infected B cells. Theresults presented here shed light on how the interactions of B. abortus with B cells may have a rolein the pathogenesis of brucellar osteoarticular disease.