Cigarette smoking, dietary habits and genetic polymorphisms in GSTT1, GSTM1 and CYP1A1 metabolic genes: A case-control study in oncohematological diseases

CERILIANI B; PAVICIC WH; GILI JA; KLEIN G,; SABA S,; RICHARD SM

World Journal Clinical Oncology

Baishideng Publishing Group Inc.

Lugar: Pleasanton; Año: 2016 vol. 7 p. 395 - 405

AIMTo analyze the association between oncohematologicaldiseases and GSTT1 /GSTM1 /CYP1A1 polymorphisms,dietary habits and smoking, in an argentine hospitalbasedcase-control study.METHODSThis hospital-based case-control study involved 125patients with oncohematological diseases and 310control subjects. A questionnaire was used to obtainsociodemographic data and information about habits.Blood samples were collected, and DNA was extractedusing salting out methods. Deletions in GSTT1 and GSTM1(null genotypes) were addressed by PCR. CYP1A1 MspIpolymorphism was detected by PCR-RFLP. Odds ratio (OR)and 95%CI were calculated to estimate the associationbetween each variable studied and oncohematologicaldisease.RESULTSWomen showed lower risk of disease compared to men(OR 0.52, 95%CI: 0.34-0.82, P = 0.003). Higher levelsof education (> 12 years) were significantly associatedwith an increased risk, compared to complete primaryschool or less (OR 3.68, 95%CI: 1.82-7.40, P < 0.001adjusted for age and sex). With respect to tobacco,none of the smoking categories showed associationwith oncohematological diseases. Regarding dietaryhabits, consumption of grilled/barbecued meat 3 ormore times per month showed significant associationwith an increased risk of disease (OR 1.72, 95%CI:1.08-2.75, P = 0.02). Daily consumption of coffee alsowas associated with an increased risk (OR 1.77, 95%CI:1.03-3.03, P = 0.03). Results for GSTT1 , GSTM1 andCYP1A1 polymorphisms showed no significant associationwith oncohematological diseases. When analyzing theinteraction between polymorphisms and tobacco smokingor dietary habits, no statistically significant associationsthat modify disease risk were found.CONCLUSIONWe reported an increased risk of oncohematologicaldiseases associated with meat and coffee intake. Wedid not find significant associations between geneticpolymorphisms and blood cancer.