Characterization and validation of FAD and SAD genes in olive (Olea europaea L.) tissue and during fruit development

CONTRERAS, ANA CIBELES; MARIOTTI, ROBERTO; CULTRERA, N; BALDONI, L; GUERRERO RUIZ, C; ROKA, L; MOUSAVI, S; PIERLUIGI PIERANTOZZI; DAMIÁN M MAESTRI; MARIELA TORRES

Rosario

Simposio; Simposio de Genómica Funcional en Plantas 2017; 2017

Stearoyl-ACPdesaturase (SADs) and fatty acid desaturases (FADs) play a critical role in plantlipid metabolism, development and acclimation to environmental stresses, andgoverning fatty acid composition of oil accumulating in seeds and fruits. In ourstudy, three SAD and four FAD candidate genes were identified in olive (Olea europaea) through blasting thegenomic scaffolds of the cv. Leccino genome sequences (http:// oleagenome.org/),followed by nucleotide BLAST in the NCBI database. Based on a phylogeneticanalysis, the three OeSADs seemed tocorrespond to SAD1, SAD2 and SAD3 proteins, sharing conserved structure withknown plant SAD proteins. FAD genescorresponded respectively to two FAD2.1, one FAD 2.2 and one FAD6. Theexpression of all genes was tested on different tissues (bud, leaf, cambium,flower, root and fruit) of cv. Leccino, showing that SAD2 andSAD3 genes were mainly expressedin fruit tissues, both FAD2.1 lociwere highly expressed in flower, whereas all the others were universallyexpressed at all tissues. Expression patterns of all genes were also observedin fruits of Leccino and Coratina varieties, both, at 45, 75, 105, 135 and 165days after flowering (DAF). Finally, considering that FAD and SAD genes show inother species a cold-induced and heat-repressed expression pattern, thepotential role of these genes, in temperature stress response, are currentlyunder analysis from three different Argentinian provinces (from the warmestCatamarca to the coldest Neuquén), during fruit development, using Arbequina asreference variety versus Coratina oneof the best olive cultivar for fatty acid composition