Genomic Insights And Biotechnological Potential Of A Multi-resistant Actinobacterium Isolated From Andean Puna Soils

ALONSO REYES, DANIEL; ALBARRACÍN, VIRGINIA H.; FARÍAS, MARÍA EUGENIA

Simposio; 16th ISCB Student Council Symposium (SCS); 2020

BACKGROUND: To enable the advance of the industrial bioprocess technology and to identify suitable new enzymes for such technology, the function, enzymatic catalog, and resistance potential of extremophiles inhabiting High-Altitude Andean Lakes (HAAL) merits investigation. Thorough genome mining, this study analyses the potential microbial functions of the polyextremophilic strain Nesterenkonia sp. Act20, a new actinobacterium isolated from the soil surrounding Lake Socompa.DESCRIPTION: Annotations unveiled the polyextremophilic nature of the Act20 genome, having the potential to resist antibiotics, arsenic, nutrient limiting conditions, osmotic stress, UV radiation, low temperatures, low atmospheric O2 pressure, heavy metal stress, fluoride, and chlorite. Also have the promise to produce or metabolize mercaptopurine, fluorouracil, a pesticide, cellulose, ectoine, colicin V and aurachin C. We also found numerous enzymes for degradation and production of compounds of interest and application in industrial processes. UV-B assays and pyrimidine photoproduct measurements showed the strain?s notable resistance to UV-B.CONCLUSIONS: This work indicates that among the extremophiles, Act20 has good potential to be a source of enzymes and compounds of biotechnological interest as well as to be a model to study the early evolution of life and a possible future terraformation of Mars.