MORPHOLOGICAL CHARACTERIZATION AND IDENTIFICATION OF BACTERIA ISOLATED FROM SURFACES AT THE “DR. CÉSAR GUERRA” CENTRAL BLOOD BANK OF TUCUMÁN

D´ARPINO, MC; ALVARADO, NN; GALVAN, S.; MARRANZINO G.; MARTINEZ, L; ALBARRACÍN V. H.

Tucuman

Jornada; XXXVIII JORNADAS CIENTÍFICAS DE LA ASOCIACIÓN DE BIOLOGÍA DE TUCUMÁN; 2021

Sociedad de Biología de Tucumán

The microbiomes of the medical-care environment play an important role as reservoirs of infectious diseases. Our goal was to studymicrobial communities at the Central Blood Bank. Surfaces from the Production and Molecular Biology services were swabbed and seededinto plates (LB pH7). The identification of the isolated strains was done by MALDI-TOF and morphological characterization by ScanningElectron Microscopy (SEM). The obtained species have clinical importance: as opportunistic pathogens (Staphylococcus haemolyticus,Staphylococcus warneri, Clostridium subterminale, Brevibacterium casei, Staphylococcus saprophyticus), multi resistance to antibiotics(Staphylococcus cohnii ssp. cohnii), spore formation (Bacillus cereus group, Bacillus sp.), or responsible for serious biofilm-relatedinfections (Micrococcus luteus, Burkholderia cenocepacia, Pseudomonas stutzeri). Some isolated bacteria are important in bioremediation(Dietzia cinnamea, Dietzia maris, Bacillus subtilis/amyloliquefaciens/vallismortis, Pseudomonas stutzeri, Bacillus altitudinis/pumilus);enzyme production (Bacillus megaterium). The analysis with MEB revealed in some strains the presence of tubular structures, possiblynanotubes, linked to biofilm production and antimicrobial resistance. Our findings show the predominant bacterial diversity in the bloodbank and describe structures possibly involved in pathogenicity mechanisms.