Magnetic Immunobiosensor coupled to carbon-based screen-printed electrodes (SPCEs) for the Botrytis cinerea determination in fruit post-harvest tissues

MARTÍN A. FERNÁNDEZ-BALDO; GERMÁN A. MESSINA; MARIA I. SANZ; JULIO RABA

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

AMER CHEMICAL SOC

Año: 2010 vol. 58 p. 11201 - 11206

0021-8561

A wide range of plant species, including economically important crops such as vegetables, ornamentals,bulbs, and fundamentally fruits, can be affected by gray mold caused by the fungal pathogenBotrytis cinerea. This paper describes the development of a microfluidic immunosensor with micromagneticbeads (MMBs) coupled to carbon-based screen-printed electrodes (SPCEs) for the rapidand sensitive quantification of B. cinerea in apple (Red Delicious), table grape (pink Moscatel), andpear (William’s) tissues. The detection of B. cinerea was carried out using a competitive immunoassaymethod based on the use of purified B. cinerea antigens immobilized on 3-aminopropyl-modifiedMMBs. The total assay time was 40 min, and the calculated detection limit was 0.008 μg mL-1.Moreover, the intra- and interassay coefficients of variation were below 7%. The developed methodallowed detects B. cinerea even in asymptomatic fruits and promises to be particularly useful forapplication in the agricultural industry.