Electrochemical immunosensing using a nanostructured functional platform for determination of a growth promoter in the livestock production

MATIAS REGIART; MARCO A. SEIA; GERMÁN A. MESSINA; FRANCO A BERTOLINO; J. RABA

MIKROCHIMICA ACTA

SPRINGER WIEN

Lugar: Viena; Año: 2015 vol. 182 p. 531 - 538

0026-3672

We describe an electrochemical immunosensor forthe determination of the growth promoter α-zearalanol inbovine serum. The sensing scheme is based on a nanocompositeconsisting of gold nanoparticles electrodeposited onmulti-walled carbon nanotubes that were modified with poly(vinylpyridine) through in-situ polymerization. The electrodepositionof the gold nanoparticles enlarges the surface availablefor immobilization of antibodies against α-zearalanol.The nanocomposite film was characterized by scanning electronmicroscopy, energy dispersive X-ray spectroscopy, andcyclic voltammetry. The calibration plot has a linear responsein the concentrations range from 0.05 to 50 ng mL−1, and thedetection limit is 16 pg mL−1. The time required for analysis is12 min only which compares quite favorably with the time(90 min) required by the conventional ELISA. The methodexhibits good selectivity, stability and reproducibility for detectingα-zearalanol in the livestock production.