Composición y estructura de la Región Control del ADN mitocondrial de camélidos sudamericanos

MATÉ M. L.; DI ROCCO F.; ZAMBELLI A.; VIDAL-RIOJA L.

Mejoramiento Genético en Camélidos Sudamericanos

CIF-FCAyP-UMSS

Año: 2003; p. 261 - 265

Owing to the economical potential of SAC (South American Camelids) as fiber producers a number of breeding and sustainable developments are getting into practice in different countries. For these to be successful it is important to know the genetic variability of the founding stocks as well as the replenishing populations. The molecular genetics is supplying useful tools to do these research among which the mitochondrial DNA D-loop has proved very much informative. In this work, we determined the composition and organization of the D-loop mtDNA from SAC and identified the most suitable region to be used in population genetics analysis. The D-loop of the four SAC species was PCR amplified, ligated to pGem T-easy vector and cloned in XL1B host. Positive colonies were sequenced in an ABI Prism 377 equipment. The results showed that the D-loop region in SAC comprise 1200 bp distributed in three domains: Conserved Sequence Blocks (CSB) 1, 2 and 3 located close to the tRNA phe gene, Central Domain (CD) and, the Extended Termination Associated Sequence (ETAS) near the tRNA pro gene. Between the CSB 1 and 2 we detected one repeated sequence 26 bp long. The alignment of all sequences from the eight SAC analyzed showed two segments 240 and 350 bases long which concentrate most of the nucleotide variability within the D-loop. We propose these segments as informative markers for analysis of population genetics variability in SAC.