IN VITRO BUENOS AIRES PARTICULATE MATTER EXPOSURE CAUSES DIRECT AND LUNG-MEDIATED INDIRECT EFFECTS ON CARDIOMYOCYTE

ORONA NS; ASTORT, F; FERRARO SA; DEBORAH R. TASAT

Bs As

Congreso; Reunión Conjunta de Sociedades de BioCiencias; 2017

Air particulate matter has been associated with adverse effects in the cardiorespiratory system leading to cytotoxic and proinflammatory effects. Particulate matter?associated cardiac effects may be direct or indirect. Direct interactions may occur if inhaled ultrafine particles and/or particle components cross the air?blood barrier while, indirect interactions may occur as the result of pulmonary inflammation and consequent release of inflammatory or oxidative mediators into the blood circulation. Therefore, we considered important to investigate the effect of Urban Air particles from downtown Buenos Aires (UAP-BA) and Residual Oil Fly Ash (ROFA), a surrogate of ambient air pollution, a) directly on cardiomyocytes (HL-1) in culture or b) indirectly by analyzing the biological response of HL-1 to alveolar macrophages (AM) particle-exposed conditioned media (CM). After 24h particle (UAP-BA or ROFA (1-200 ug/mL)) or CM exposure, cultured cells we assessed for: viability by MTT, Reactive oxygen species (ROS) by DHR 123 and NRF-2 expression by immunocytochemestry. We found that a) Cardiomyocytes exposed directly to UAP-BA or ROFA showed a marked increase in ROS generation and NRF2expression, without alterations in cell viability. When b) UAP-BA or ROFA indirect effect was assayed on HL-1 cells, we observed that CM provoke a reduction in cell viability (CCM:1.255±0.086 vs ROFA1CM:0.941±0.096, ROFA10CM:1.016±0.064, ROFA100CM:0.464±0.013, UAP-BA10CM:1.016±0.064, UAP-BA100CM:0,676±0.034, UAP-BA200 CM:0.725±0.017 ABS, p