N-ACETYL CYSTEINE PREVENTS BUENOS AIRES URBAN AIR PARTICLES ADVERSE EFFECTS ON MICE LUNG AND LIVER

EDUARGO G AGOSTA; NADIA S. ORONA; DEBORAH R. TASAT; FRANCISCO ASTORT

Bs As

Congreso; Reunión Conjunta de Sociedades de BioCiencias; 2017

Particulate matter (PM) is one of the main air environmental pollutants. Epidemiological studies have shown that PM can increase cardiorespiratory morbimortality, being the lungs the main PM body entrance target organ. Ultrafine particles (PM 0.1), because of their size, can translocate from the lung into the circulation affecting other body organs. Among them, the liver plays a key role in xenobiotics metabolism and its malfunction has been associated with increased cardiovascular risk. Previous work from our group demonstrated that Urban Air Particles from Buenos Aires (UAP-BA) induces in lung, inflammation and oxidative stress. Therefore, the aim of this study was to analyze the influence of the antioxidant, N-acetyl cysteine (NAC), on UAP-BA acute impact on mice lung and liver. BALB/c mice (N=20) were i.p. injected with NAC (150 mM/kg BW) or vehicle, and 24h later exposed by intranasal instillation to UAP-BA (1 mg/kg BW) or vehicle. We evaluated 24h post-instillation the total cell number (TCN), and superoxide anion generation (O2-) by NBT in bronchoalveolar lavage fluid (BAL) and, superoxide dismutase (SOD) and catalase (CAT) activities spectrophotometrically, reduced glutathione (GSH) by DTNB and lipoperoxide levels by TBARS in liver homogenates.Exposure to UAP-BA increased TCN and O2- production in BAL (p