Oxidative metabolism study after particle air pollution exposure in a chronic nutritional stress model

KURTZ M; ASTORT F; CHRISTIAN E. LEZÓN; CHAMPIN G; FERRARO SA; FRIEDMAN S; DR TASAT; PATRICIA M. BOYER

MDQ

Congreso; SAIC ? SAI ? SAFE 2016; 2016

Air particulate pollution negatively affects the health of the population exposed, been the lung the main target organ. Evidences of the interrelationship between nutritional status and oxidative stress and inflammation caused by air pollution have been documented. Previously, we demonstrated homeostatic stress systems activation in growing rats, under chronic nutritional stress (CNS). However, there is no evidence of adverse outcomes due to pollution environment exposition in this CNS experimental model. The aim of this work was to study in vivo, in growing animals under chronic nutritional stress, the oxidative metabolism after acute exposure to Residual Oil Fly Ash-ROFA, a known surrogate ambient air particle pollution, Twenty-eight weanling male Wistar rats were randomized in two groups regarding food intake: 1) ad libitum foodintake (Control-C), or 2) 80% of the amount of food consumed by control (Chronic Nutritional Stress-CNS). After 4 weeks animals were intranasally instilled either with ROFA (1mg/kg BW) or saline solution defining 4 groups: C, CNS, C+ROFA, CNS+ROFA. After 24h,broncheo-alveolar lavage fluid was obtained and the following parameters were examined: Total Cell Number (TCN), Differential Cell Count (CCD) and superoxide anion (O2-.) generation. In lung homogenates superoxide dismutase (SOD) and catalase (CAT) antioxidant enzymes activity were determined. In blood the number of white cells (BWC) was determined as number of cells/mm3. Results: TCN augmented after ROFA exposure being only significant for C+ROFA group (C:6.9±1.5 vs C+ROFA: 17.0±3.1.p