Hepatotoxicity induced by Acute Exposure to Buenos Aires Urban Air Particles and Residual Oil Fly Ash in Young and Middle-Aged Mice

ORONA NS; ASTORT F; FERRARO SA; MAGLIONE GA; MARTIN M; FERNANDO BRITES; MANDALUNIS PM; CELINA MORALES; TASAT DR

Mar del Plata

Congreso; SAIC ? SAI ? SAFE 2016; 2016

Adverse health effects of air particulate matter (PM) are associated to respiratory and cardiovascular diseases. PM doesn?t affect equally to all people, being life stage a putative parameter that can influence PM-related health effects. Regarding particle size, ultrafine particles can be translocated from the lungs into the circulation and extrapulmonary organs among which the liver is included. Liver plays a central role in the metabolism and excretion of xenobiotics which makes it highly susceptible to their adverse and toxic effects. The aim of this study was to analyze in the liver of young and middle-aged mice the acute biological impact of Urban Air Particles from Buenos Aires (UAP-BA) in comparison with Residual Oil Fly Ash (ROFA), an ambient particle surrogate. Young (3 month old) and middle-aged (10 months old) BALB/c mice were exposed by intranasal instillation with UAP-BA or ROFA (1mg/kg BW). We evaluated at 3, 24 or 48h post-instillation liver morphology, alanine aminotransferase (ALT) and aspartate aminotransferase (AST), two serum enzymatic biomarkers indicative of hepatocellular toxicity. In young mice, UAP-BA and ROFA induced serum AST activity augmentation only at 3h post-instillation when compared to controls (C:149.4±44.3 vs. UAP-BA3:700.4±452.6, ROFA3:365.2±112.8 U/L, p