Differential susceptibility to particulate ambient air pollution exposure in a chronic nutritional stress model

ASTORT F1, LEZON CE2, CHAMPIN G2, ORONA NS1, FERRARO SA1, FRIEDMAN SM3, BOYER PM2, TASAT DR1,4.

BS AS

Congreso; SETAC Latin America 11th Biennial Meeting; 2015

SETAC LA

Introduction: Air particulate pollution negatively affects the health of the population exposed, been the lung the main target organ. Evidences of the interrelationship between nutritional status and oxidative stress and inflammation caused by air pollution have been documented. Previously, we demonstrated homeostatic stress systems activation in growing rats, under chronic nutritional stress. However, in the present model there is no evidence of adverse outcomes due to pollution environment exposition.Objective: The aim of this study was to assess the susceptibility to particulate ambient air pollution (Residual Oil Fly Ash-ROFA) exposure in growing animals under chronic nutritional stress.Materials and Methods: Twenty-eight weanling male wistar rats were randomized in two groups regarding food intake: 1) ad libitum food intake (Control-C), or 2) 80% of the amount of food consumed by control (Chronic Nutritional Stress-CNS). After 4 weeks animals were intranasally instilled either with ROFA (1mg/kg BW) or saline solution defining 4 groups: C, CNS, C+ROFA, CNS+ROFA. After 24h, broncheo-alveolar lavage fluid was obtained and the following parameters were examined: Total Cell Number (TCN), DifferentialCell Count (CCD) and superoxide anion (O2-.) generation.Results: TCN augmented after ROFA exposure being only significant for C+ROFA group (C: 6.9±1.5 vs C+ROFA: 17.0±3.1.p