EFFECTS OF BACTERIAL LIPOPOLYSACCHARIDE ON CICLOOXYGENASE ACTIVITY AND EXPRESSION LEVELS AND STEROID PRODUCTION IN ADRENAL Y1 CELLS: POSSIBLE INVOLVEMENT OF NFB SIGNALING

MARTINEZ CALEJMAN C; ASTORT F; DI GRUCCIO JM; REPETTO EM; MERCAU M; ARIAS P; PIGNATARO O; CYMERYNG, C

Rio de Janeiro, Brasil

Congreso; International congress of endocrinology (ISE); 2008

international society of endocrinology (ISE)

Although the increase in glucocorticoid secretion by the adrenal cortex during the inflammatory response to systemic lipopolysaccharide (LPS) administration has been mainly attributed to effects at higher levels of the hypotalamic-pituitary-adrenal (HPA) axis, increasing evidence supports a direct effect of LPS on adrenocortical cells. In this sense, the aim of this study was to analyze the effects of LPS on steroid production by adrenal Y1 cells, as well as the signaling/transduction mechanisms involved. According to our results, this murine adrenal cell line expresses Toll-like 4 receptors, demonstrated by RT-PCR. When  Y1 cells were transfected with a luciferase reporter plasmid containing an NFkB response element, LPS stimulation (10 µg/ml)  resulted in a significant increase in luciferase activity (C: 0,88 ± 0,10; LPS: 2,00 ± 0,09; p