Lung and keratinocyte cells differencial response to rural air particulate matter

NADIA S. ORONA; ASTORT, FRANCISCO; FENOY IGNACIO; IMANOL PALAVECINO; RAMIREZ HABERKON NANCY; MENDEZ MARIANO; RANDI ANDREA; JUAN ESTEBAN PANEBIANCO; GOLDMAN ALEJANDRA

Congreso; Reunión Conjunta SAIC . SAI . AAFE . NANOMER.ar; 2021

Atopic disorders susceptibility depends on interactions between genetic and environmental factors. The prevalence of these disorders has increased in the last 5 decades indicating that environmental factors are responsible for this increase. Airborne particulate matter (PM) is one of the main environmental health problems affecting the world´s population. Although several studies have related the exposure to urban PM with the onset or exacerbation of allergies such as asthma and atopic dermatitis, there are few studies that link rural PM with the development of these allergic disorders. Rural PM comes mainly from agricultural activity, where agrochemical compounds are frequently used. Although agrochemical exposure has been related to allergic diseases, an approach that considers the synergistic effects of the interaction of PM and agrochemicals is necessary. Therefore, the aim of this study was to evaluate the effect of Rural PM from Pampeana region on pulmonary (A549) and skin (HaCaT) cells. A549 or HaCaT cells were exposed to soil generated-PM10 (1, 10 and 100µg/ml) from an intensive cultivation area where the use of agrochemicals prevails, or glyphosate (0.75, 7.5, 75 and 7500 pg/ml). After 24h, cell metabolic activity, lactate dehydrogenase (LDH) and cytokine release (IL-8, TSLP, IL-1β) was assessed. We found that A549 cells exposed to PM showed a marked increase in IL-8 production (P