INVESTIGADORES
FERNANDEZ Paula Del Carmen
artículos
Título:
Cytological characterization of sunflower by in situ hybridization using homologous rDNA sequences and a BAC clone containing highly represented repetitive retrotransposon like sequences
Autor/es:
P. TALIA; E. GREIZERSTEIN; D. DÍAZ QUIJANO; L. PELUFFO; L. FERNANDEZ; P. FERNANDEZ; H.E. HOPP; N. PANIEGO; R.A. HEINZ; L. POGGIO
Revista:
GENOME
Editorial:
NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS
Referencias:
Año: 2010 vol. 53 p. 172 - 179
ISSN:
0831-2796
Resumen:
Abstract: In the present work we report new tools for the characterization of the complete chromosome complement of
sunflower (Helianthus annuus L.), using a bacterial artificial chromosome (BAC) clone containing repetitive sequences
with similarity to retrotransposons and a homologous rDNA sequence isolated from the sunflower genome as probes for
FISH. The rDNA signal was found in 3 pairs of chromosomes, coinciding with the location of satellites. The BAC clone
containing highly represented retroelements hybridized with all the chromosome complement in FISH, and used together
with the rDNA probe allowed the discrimination of all chromosome pairs of sunflower. Their distinctive distribution pattern
suggests that these probes could be useful for karyotype characterization and for chromosome identification. The karyotype
could be subdivided into 3 clear-cut groups of 12 metacentric pairs, 1 submetacentric pair, and 4 subtelocentric
pairs, thus resolving previously described karyotype controversies. The use of BAC clones containing single sequences of
specific markers and (or) genes associated with important agricultural traits represents an important tool for future locusspecific
identification and physical mapping.
with similarity to retrotransposons and a homologous rDNA sequence isolated from the sunflower genome as probes for
FISH. The rDNA signal was found in 3 pairs of chromosomes, coinciding with the location of satellites. The BAC clone
containing highly represented retroelements hybridized with all the chromosome complement in FISH, and used together
with the rDNA probe allowed the discrimination of all chromosome pairs of sunflower. Their distinctive distribution pattern
suggests that these probes could be useful for karyotype characterization and for chromosome identification. The karyotype
could be subdivided into 3 clear-cut groups of 12 metacentric pairs, 1 submetacentric pair, and 4 subtelocentric
pairs, thus resolving previously described karyotype controversies. The use of BAC clones containing single sequences of
specific markers and (or) genes associated with important agricultural traits represents an important tool for future locusspecific
identification and physical mapping.
sunflower (Helianthus annuus L.), using a bacterial artificial chromosome (BAC) clone containing repetitive sequences
with similarity to retrotransposons and a homologous rDNA sequence isolated from the sunflower genome as probes for
FISH. The rDNA signal was found in 3 pairs of chromosomes, coinciding with the location of satellites. The BAC clone
containing highly represented retroelements hybridized with all the chromosome complement in FISH, and used together
with the rDNA probe allowed the discrimination of all chromosome pairs of sunflower. Their distinctive distribution pattern
suggests that these probes could be useful for karyotype characterization and for chromosome identification. The karyotype
could be subdivided into 3 clear-cut groups of 12 metacentric pairs, 1 submetacentric pair, and 4 subtelocentric
pairs, thus resolving previously described karyotype controversies. The use of BAC clones containing single sequences of
specific markers and (or) genes associated with important agricultural traits represents an important tool for future locusspecific
identification and physical mapping.
with similarity to retrotransposons and a homologous rDNA sequence isolated from the sunflower genome as probes for
FISH. The rDNA signal was found in 3 pairs of chromosomes, coinciding with the location of satellites. The BAC clone
containing highly represented retroelements hybridized with all the chromosome complement in FISH, and used together
with the rDNA probe allowed the discrimination of all chromosome pairs of sunflower. Their distinctive distribution pattern
suggests that these probes could be useful for karyotype characterization and for chromosome identification. The karyotype
could be subdivided into 3 clear-cut groups of 12 metacentric pairs, 1 submetacentric pair, and 4 subtelocentric
pairs, thus resolving previously described karyotype controversies. The use of BAC clones containing single sequences of
specific markers and (or) genes associated with important agricultural traits represents an important tool for future locusspecific
identification and physical mapping.
In the present work we report new tools for the characterization of the complete chromosome complement of
sunflower (Helianthus annuus L.), using a bacterial artificial chromosome (BAC) clone containing repetitive sequences
with similarity to retrotransposons and a homologous rDNA sequence isolated from the sunflower genome as probes for
FISH. The rDNA signal was found in 3 pairs of chromosomes, coinciding with the location of satellites. The BAC clone
containing highly represented retroelements hybridized with all the chromosome complement in FISH, and used together
with the rDNA probe allowed the discrimination of all chromosome pairs of sunflower. Their distinctive distribution pattern
suggests that these probes could be useful for karyotype characterization and for chromosome identification. The karyotype
could be subdivided into 3 clear-cut groups of 12 metacentric pairs, 1 submetacentric pair, and 4 subtelocentric
pairs, thus resolving previously described karyotype controversies. The use of BAC clones containing single sequences of
specific markers and (or) genes associated with important agricultural traits represents an important tool for future locusspecific
identification and physical mapping.
with similarity to retrotransposons and a homologous rDNA sequence isolated from the sunflower genome as probes for
FISH. The rDNA signal was found in 3 pairs of chromosomes, coinciding with the location of satellites. The BAC clone
containing highly represented retroelements hybridized with all the chromosome complement in FISH, and used together
with the rDNA probe allowed the discrimination of all chromosome pairs of sunflower. Their distinctive distribution pattern
suggests that these probes could be useful for karyotype characterization and for chromosome identification. The karyotype
could be subdivided into 3 clear-cut groups of 12 metacentric pairs, 1 submetacentric pair, and 4 subtelocentric
pairs, thus resolving previously described karyotype controversies. The use of BAC clones containing single sequences of
specific markers and (or) genes associated with important agricultural traits represents an important tool for future locusspecific
identification and physical mapping.
Helianthus annuus L.), using a bacterial artificial chromosome (BAC) clone containing repetitive sequences
with similarity to retrotransposons and a homologous rDNA sequence isolated from the sunflower genome as probes for
FISH. The rDNA signal was found in 3 pairs of chromosomes, coinciding with the location of satellites. The BAC clone
containing highly represented retroelements hybridized with all the chromosome complement in FISH, and used together
with the rDNA probe allowed the discrimination of all chromosome pairs of sunflower. Their distinctive distribution pattern
suggests that these probes could be useful for karyotype characterization and for chromosome identification. The karyotype
could be subdivided into 3 clear-cut groups of 12 metacentric pairs, 1 submetacentric pair, and 4 subtelocentric
pairs, thus resolving previously described karyotype controversies. The use of BAC clones containing single sequences of
specific markers and (or) genes associated with important agricultural traits represents an important tool for future locusspecific
identification and physical mapping.