Characterization of a human apolipoprotein A-I construct expressed in a bacterial system

EDUARDO DANIEL PRIETO, NAHUEL RAMELLA, LUZ ANGELA CUELLAR, MARÍA ALEJANDRA TRICERRI, HORACIO ALBERTO GARDA

PROTEIN JOURNAL

SPRINGER

Lugar: Berlin; Año: 2012 p. 1 - 8

1572-3887

Apolipoprotein A-I (apoA-I) is the major protein component of high density lipoproteins. This protein has key functions in lipoprotein metabolism and its plasma concentration is inversely correlated with the incidence of atherosclerosis and cardiovascular diseases. There is an increasing need to develope methods for efficient production of recombinant apoA-I for using it in basic research or pharmacological theraphy. An apoA-I variantlacking two amino acid residues at the Nterminal can be easily produced by bacterial expression. We report herethe characterization of this variant comparing its properties with those of the protein isolated from human serum. The results validate the use of this variant in future assays and investigations