Effect of Yerba Mate (Ilex paraguariensis) on Osteoblastic Cells

BRUN LR; VILLAREAL L; RICO MJ; ROZADOS VR; SCHAROVSKY OG; DI LORETO VE

Denver

Congreso; American Society for Bone and Mineral Research Meeting 2017; 2017

American Society for Bone and Mineral Research

Yerbamate (I. paraguariensis) infusion isfrequently drank in several Latin American countries such as Uruguay, Argentina,Brazil and Paraguay. Several active phytochemicals including methyl xanthines(caffeine) and polyphenols have been identified in yerba mate. Caffeine hasshown a negative impact on BMD in particular when it is associated with lowcalcium diet. Polyphenols have shown positive bone effects due to theirantioxidant properties. An increased lumbar spine and femoral neck BMD havebeen demonstrated in postmenopausal women who drank at least 1 liter of I. paraguariensis infusion per day. Moreover,in rats the I. paraguariensisinfusion increased the BMD and the trabecular bone volume with no effect onbiomechanical properties. The aim of this study was to measure the content of I. paraguariensis components withpotential effect on bone tissue and to evaluate the effect of these componentson osteoblastic cells. Caffeine, total polyphenols, calcium, phosphate andfluoride content were measured in 12 commercial trademarks of I. paraguariensis infusion (triplicates). It was calculated the total polyphenols concentrationthat could scavenge 50% of the 2,2-difenil-1-pricril-hidrazil radical (IC50)in order to measure the antioxidant activity. In addition, the effect ofcaffeine and polyphenols (rutin, quercetin and chlorogenic acid) on MC3T3E1osteoblastic cells viability (Cell Proliferation Reagent WST-1, Roche) wereevaluated. No differences in caffeine (95%CI 0.59-0.83 g/l), total polyphenols (14.5-16.0g/l), calcium (13.3-16.7 mg/l), phosphate (101.3-135.7 mg/l) and fluoride (0.066-0.070mg/l) content were found between the different commercial trademarks of I. paraguariensis evaluated. Nodifferences were observed either in antioxidant activity (IC50 64.8-71.6µg/ml). A significant increase in osteoblastic cells viability was observed atdifferent concentrations (1 to 10 μg/ml) of rutin (~26%) and caffeine (~34%) compared with cells without treatment(controls). A significant increase on viability was also observed with 1 to 5 μg/mlof chlorogenic acid (~27%)and quercetin (~16%).Therefore, it is concluded that there were no significant differences betweenthe different trademarks analyzed in their content of components with potentialeffect on bone tissue or its antioxidant activity. Our data suggest that thepositive skeletal effects of I.paraguariensis could be attributed, at least in part, to stimulation ofosteoblasts survival