Sequential treatment with monofluorophosphate and zoledronic acid in osteoporotic rats

BRANCE ML; BRUN LR; DI LORETO VE; LUPO M; RIGALLI A

CLIMACTERIC

TAYLOR & FRANCIS LTD

Lugar: Londres; Año: 2014 vol. 74 p. 478 - 485

1369-7137

Osteoporosis is the consequence of an imbalance in bone remodeling caused by excessive resorption or inappropriate bone formation. This paper proposed a sequential treatment with monofluorophosphate (MFP) and zoledronic acid (Z), together with changes in the calcium (Ca) content in the diet. Five experimental groups were performed: Sham: sham-operated rats; OVX: ovariectomized (OVX) rats fed with normal Ca diet; OVX.G1: OVX rats fed with normal Ca diet, treated sequentially with MFP and Z; OVX.G2: OVX rats sequentially fed with low Ca diet and high Ca diet, without treatment; OVX.G3: OVX rats fed with low Ca diet and high Ca diet, treated sequentially with MFP and Z. After 150 days, OVX.G3 group showed similar bone volume compared with Sham group due to an increase in trabecular number. Dual X-ray absorptiometry bone analysis showed an increase of 9.8% compared with OVX. Additionally, an increase in the fracture load at the cortical bone and higher fracture load, ultimate load and stiffness in the compression test was found. In summary, the sequential treatment with MFP and Z increases trabecular bone mass, bone mineral density and bone strength.