INVESTIGADORES
BRUN Lucas Ricardo Martin
artículos
Título:
Effect of calcium on rat intestinal alkaline phosphatase activity and molecular aggregation
Autor/es:
BRUN LR; BRANCE ML; RIGALLI A; PUCHE RC
Revista:
Journal of enzyme inhibition and medicinal chemistry
Editorial:
Taylor & Francis Group
Referencias:
Lugar: Abingdon (UK); Año: 2006 vol. 21 p. 757 - 763
ISSN:
1475-6366
Resumen:
Two fractions of rat intestinal alkaline phosphatase (IAP) were detected by Western blot: 168±6 and 475±45 kDa. The low molecular weight fraction constitutes 43% of the isolated proteins exhibiting 82% of the enzymatic activity, and a heavier fraction constitutes 57% of the isolated proteins and has 18% of the enzymatic activity. Calcium produced increased of 475-kDa form to the detriment of 168-kDa form. This work also describes kinetic and structural changes of IAP as a function of calcium concentration ([Ca2+]). With [Ca2+]
