EXPRESSION ANALYSIS OF GENES POTENTIALLY INVOLVED IN INDUCED RESISTANCE MEDIATED BY PHOSPHITE IN POTATO

FELDMAN ML; MACHINANDIARENA MF; DALEO GR; ANDREU AB

Potrero de los Funes, San Luis

Congreso; XLVII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2011

Sociedad Argentina de Investigación en Bioquímicay Biología Molecular

Phosphite (Phi) compounds have the ability to protect plants against different pathogens. Treatments with Phi could be used in an integrated crop management program as an alternative that would reduce the use of fungicides during the crop cycle. We have shown through microarray analysis, that in potato sprouts treated with potassium phophite (KPhi), 26 genes were upregulated compared with non treated ones. Within those we have selected 3 genes (Cullin1, ERD15 & BCYA) known to be involved in induced resistance (IR) and we validated them by qRT PCR. The aim of this work was to analyse their expression in leaves treated or not with KPhi, infected or not with Phytophtora infestans (Pi). Semi quantitative RT PCR showed that the 3 genes were expressed only in KPhi treated leaves. To analyze if Pi infection acts sinergically with KPhi, we quantify their expression 24 and 48 h after infection, in KPhi treated or non treated leaves. After 24 h, BCYA and Cullin 1 increased their expression in infected leaves, with respect to the non infected ones, however KPhi treatment did not act sinergically, moreover it was reduced for Cullin 1. ERD15 expression did not change with respect to the non infected leaves, but it increased when KPhi was applied. Their expression decreased after 48 h treatments. These results could help to understand the battery of responses involved in IR mediated by KPhiPhytophtora infestans (Pi). Semi quantitative RT PCR showed that the 3 genes were expressed only in KPhi treated leaves. To analyze if Pi infection acts sinergically with KPhi, we quantify their expression 24 and 48 h after infection, in KPhi treated or non treated leaves. After 24 h, BCYA and Cullin 1 increased their expression in infected leaves, with respect to the non infected ones, however KPhi treatment did not act sinergically, moreover it was reduced for Cullin 1. ERD15 expression did not change with respect to the non infected leaves, but it increased when KPhi was applied. Their expression decreased after 48 h treatments. These results could help to understand the battery of responses involved in IR mediated by KPhi