Ovarian mononuclear cells distribution and its influence on pathogenesis of bovine cystic ovarian disease (COD)

STASSI AF; ETCHEVERS L; CAINELLI S; AMWEG AN; ORTEGA HH; REY F; SALVETTI NR

Congreso; Reunión Conjunta SAIC SAI AAFE NANOMED; 2021

COD results fromfailure in the ovulation and the study of the processes that lead to thisfailure and persistence of the dominant follicle in the ovary is the key to understandthe pathogenesis of COD in cattle. Ovulation has been characterized as an inflammatoryprocess and mononuclear cells (MC) participate in this process, therefore, theaim of this study was to evaluate the populations of macrophages (CD14+), T(CD2+) and B (CD79+) lymphocytes in ovaries of animals with induced follicularpersistence and spontaneous COD (sCOD). Ovariectomy was performed to obtainovaries with sCOD (n = 5). Also, an experimental model of follicularpersistence was performed, with an intravaginal progesterone (P4) device to getsubluteal concentrations of P4, obtaining dominant follicles around ovulation(n = 5; P0) and follicles that persist for 5 (n = 5; P 5), 10 (n = 5; P10) or15 days (n = 5; P15) after the expected time of ovulation. Controls cows wereovariectomized in proestrus (n = 5; C). MC populations were evaluated throughimmunohistochemistry in ovarian cortex, medulla, theca interna and externa ofpersistent follicles, cysts and dominant. The specificity of the antibodies wascorroborated by western blot. The data were analyzed through ANOVA with Duncanpost-test to contrast the evaluated structures of the experimental model and Cgroup, and T-student test for sCOD and C group. The number of CD14+, CD2+ andCD79+ cells was higher in ovarian cortex, medulla, theca interna and externa ofC group than in sCOD, P0, P10 and P15 groups (p<0.05). Exceptionally, CD79+cells in the ovarian cortex of the P0 group were similar respect to the C group(p>0.05). Because these populations constitute potentialin situ modulators of ovarian function, acting through the secretion ofregulatory factors of the inflammatory process related to ovulation, we proposethat this low proportion of these cells could be partly responsible for theanovulation observed in COD.