Alteration of key metabolic components in a follicular persistence model in dairy cows

GAREIS NC; CATTANEO MOREYRA ML; DÍAZ PU; ORTEGA HH; SALVETTI NR; HEIN GJ; REY F

Congreso; Reunión Conjunta SAIC SAI AAFE NANOMED; 2021

Severallocal metabolic factors are involved in the ovarian follicular persistenceassociated with anovulation similar to the ovaries from cows with cysticovarian disease. Therefore, we aimed to study the protein expression ofAdipoR1, AdipoR2, AMPK, CPT1 and ACOX1 in ovarian follicular structures duringthe development of follicular persistence in cows. Non-lactating Holstein cows(n=25) with regular estrous cycles without previous reproductive disease werestudied. The persistence model was performed with an intravaginal progesterone deviceto get subluteal concentrations of progesterone, obtaining dominant folliclesby ovariectomy at the expected day of ovulation (n=5; P0) and follicles thatpersist for 5 (n=5; P5), 10 (n=5; P10) or 15 days (n=5; P15) relative to theexpected time of ovulation. Controls cows, without progesterone treatment, wereovariectomized in proestrus (n=5; C). Expression of target protein was assessedby indirect immunohistochemistry in ovarian tissue sections. In theca cells,AdipoR1 expression was higher in persistent follicles of P15 thanpersistent/dominant follicles of other groups (p<0.05). AdipoR2 expressionin theca cells was higher in persistent follicles of P15 than follicles ofgroups C and P0 (p<0.05). AMPK expression tends to increase (p=0.078) in thegranulosa cells of the persistent follicles of the P15 group with respect topersistent/dominant follicles of the other groups. In granulosa cells, theexpression of CPT1 was higher in persistent follicles of P5, P10 and P15 groupsthan C and P0 groups (p<0,05). In theca cells, the expression was higher inpersistent follicles of P15 than C, P0 and P5 groups (p<0.05). ACOX1 expressionshowed no differences (p>0.05) in follicular structures analyzed. Theseresults evidence a local alteration in some metabolic sensors from the initialstages of persistence, possibly related to alterations in the oxidation offatty acids at intraovarian level.