Effects of mineral and vitamin supplementation in the oxidative stress biomarker 3-nitrotyrosine in plasma, urine and liver tissue of dairy cattle during the transition period

ANGELI E; BARCAROLO D; BIANCIOTTI LG; REY F; ORTEGA HH; HEIN GJ

Mar de Plata

Congreso; Reunión Conjunta SAIC SAI AAFE NANOMED; 2021

SAIC, SAI, AAFE, NANOMED

Theintensification of milk production has generated a great metabolic demand inthe animals causing an increase in the oxidative stress, particularly duringthe transition period of dairy cattle. Therefore, we aimed to study theconcentration of free 3-nitrotyrosine (3- NT) in plasma, urine and livertissue, as a marker of the oxidative stress, and other metabolic biomarkers inplasma of cows supplemented with vitamins and minerals during the transitionperiod. The supplemented group (SG; n = 11) received subcutaneously a dose of 5ml of the vitamin supplement ADAPTADOR® Vit and 5 ml of the mineral supplementADAPTADOR® Min (Biogenesis Bagó, Bs. As.; vitamin A palmitate 3.5% and vitaminE acetate 5%, copper edetate 1%, zinc edetate 4%, manganese edetate 1% andsodium selenite 0.5%) on -60, -30 and 7 days relative to calving. The control group(CG; n = 11) received two injections of 5 ml of 0.9 % sodium chloride. Blood,urine and liver biopsies were sampled at -21, 7 and 21 days relative to calvingand to evaluate 3-NT. Also, plasma concentrations of non-esterified fattyacids, beta-hydroxybutyrate, glucose, albumin, cholesterol, bilirubin,aspartate aminotransferase, gamma-glutamyl transferase and livertriacylglycerol content were spectrophotometrically measured. In cows of theSG, 3-NT concentration was greater in plasma (p < 0.05) and lesser in theliver tissue (p < 0.05). In addition, a lesser liver triacylglycerol contentand greater glucose concentration was detected in cows of the SG (p < 0.05).Regarding albumin, plasma concentration was greater in cows of the SG (p <0.05), with a supplementation x time effect (p < 0.05), being particularlygreater on day 21 pre-calving and day 4 post-calving. No statisticaldifferences were evidenced in the other studied parameters. These results suggestthat mineral and vitamin supplementation could ameliorate the oxidative stressin the liver and the transition of dairy cows.