Endometrial inflammatory cytokines: the effects of milk production levels and hormonal context on local expression of them during early lactation of dairy cattle

PERALTA MB; ANGELI E; STASSI AF; CAINELLI S; DURANTE LN; ORTEGA HH; REY F; SALVETTI NR; VELÁZQUEZ MML

Mar del Plata

Congreso; LXII Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2018

Dairycattle undergo metabolic and hormonal changes that impact in the immunologicalsystem during peripartum. Previous reports suggest that cows areimmunosuppressed during postpartum and immune components of the reproductivetract are altered. We analyze the impact of milk production and the hormonalcontext on the expression of endometrial cytokines. The animals were divided inhigh production group (HP: > 4800 L, n = 11) and low production group (LP:< 4400 L, n = 13) considering the average of the dairy production up to 150days in lactation. Blood samples and endometrial biopsies were obtained at 45and 60 days postpartum (dpp). The mRNA expression of interleukin IL1α,TNFαand IL6 was evaluated by real time PCR. The protein expression was analyzed by immunohistochemistryin the endometrial luminal epithelium (LE), glandular epithelium (GE) andstroma (S). Plasmatic concentrations of cortisol, estrogen and progesteronewere measured by ECLIA. A general linear model was used to analyze the results.The results showed that mRNA levels of IL1α were lower at 60 dpp than 45 dpp,and LP group showed a decrease of IL1α mRNA expression in relation to HP group(p<0,05). Also, the estradiol levels had significant effect on the increaseof IL1α mRNA expression. The mRNA levels of TNFα showed no differences betweenanalyzed groups and the 75% of the samples showed no expression of IL6 mRNA. Theprotein expression of IL1α was significantly increased in LE under progesteroneeffect (p<0,05). TNFα showed no differences in LE, GE and S between HP andLP groups (p>0,05). However, the protein levels of IL6 in GE weresignificantly increased under the progesterone effect. These results suggestthat milk production and the hormonal context affect the uterine cytokineexpression during evaluated postpartum period, especially under estradiol andprogesterone effects.