Alterations of adiponectin receptors and 5-AMP kinase expression in follicular structures of cows with cystic ovarian disease.

GAREIS NC; DÍAZ PU; SALVETTI NR; PANZANI CG; ORTEGA HH; HEIN GJ; REY F

Mar del Plata

Congreso; Reunión anual de SAIC, SAI, SAFE; 2016

Adiponectin is an adipokine produced by adipose tissue which regulates several reproductive processes. Its role in steroidogenesis, considering the energy balance for the ovary functions,could be potentially associated to the development of reproductive disorders such as cystic ovarian disease (COD). The aim of this study was to determine the expression of components of the adiponectin system in ovarian follicles of cows with spontaneous COD and control cows. Thus, the receptors of adiponectin, AdipoR1 and AdipoR2, and the enzyme 5-AMP kinase (AMPK) were evaluated. Animalswith spontaneous COD (n=8) and control cows (n=8) were ovariectomized and ovaries obtained were processed histologically to embedded in paraffin. Expression of target protein was assessed by indirect immunohistochemistry on ovarian tissue sections. Results showed similar expression of AdipoR1 infollicular structures evaluated in ovaries from control cows and cows with COD, showing a tendency to decrease the expression in granulosa cells of cysts regarding to antral follicles (as reference structure) of control cows (p<0.1). However, a higher expression of AdipoR2 was detected in theca cells of cysts compared to antral follicles of control cows (p<0.05) without differences in granulosa cells. Moreover, the expression of AMPK in theca cells of cysts was higher compared to control antral follicles (p<0.05) without differences in granulosa cells of the follicles analyzed. The results of the present study showed variations in proteins involved in response and signaling to adiponectin. Considering the association of this adipokine with several metabolic functions, such as improving the insulin sensitivity, and also its essential role for follicular growth and maturation, the alterations here detected could indicate a potential contribution of this cytokine in ovarian function relatedto reproductive disorders.