Altered expression of steroid hormones receptors coregulators during bovine cystic ovarian disease development

NOTARO US; HUBER E; SALVETTI NR; ORTEGA HH; REY F; RODRÍGUEZ FM; RECCE S; BARBERIS F

Mar del Plata

Congreso; Reunión anual de SAIC, SAI, SAFE; 2016

Steroidhormones regulate important reproduction events through its nuclear receptors.Steroid hormones receptors are ligand regulated transcription factors whichmodulate genic transcription through molecular mechanisms associated withcoregulatory proteins (coactivators and corepresors). The endocrine profile, growthdynamics, and histologic characteristics of persistent ovarian follicles areanalogous to those of spontaneous cysts. Cystic Ovarian Disease (COD) is amajor factor contributing to poor reproductive efficiency of lactating dairycow. Our purpose was to study the protein expression of Steroid ReceptorCoactivator-1 (SRC-1) and corepressors Receptor-interacting Protein 140 (RIP140)and Ligand dependent nuclear receptor Corepressor (LCOR) in the ovaries ofhealthy cows (Control group) and in an experimental model of follicularpersistence induced by low levels of progesterone. This was achieved byindirect immunohistochemistry and digital image analysis of granulosa cells(GC) and theca cells (TC) in different follicular categories: antral folliclesof the control group as reference structure (AC), persistent follicles of groupswith 0 (P0, expected day of ovulation), 5 (P5), 10 (P10) and 15 (P15) days ofpersistence. Expression of SRC-1 was lower in AC than in P0 and P15, in both GCand TC (p<0.05). Expression of LCOR was higher in GC of AC and P0 than P10(p<0.05), with no differences in TC (p>0.05). Expression of RIP140 wassimilar in all analyzed follicular categories (p>0.05), in both GC and TC. Theseresults suggest that changes in the expression of coregulatory proteins canlead to altered response to steroid hormones, and thus contribute to thepathogenesis of ovarian alterations such as follicular persistence and COD.