INVESTIGADORES
REY Florencia
artículos
Título:
Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution
Autor/es:
DALLARD BE; PUJATO S; BARAVALLE C; PEREYRA EAL; REY F; RENNA MS; ORTEGA HH; CALVINHO LF
Revista:
RESEARCH IN VETERINARY SCIENCE
Editorial:
ELSEVIER SCI LTD
Referencias:
Año: 2013 vol. 94 p. 462 - 470
ISSN:
0034-5288
Resumen:
The objective
of this study was to evaluate the effects of a single intramammary infusion of Panax
ginseng extract (GS) on insulin-like growth factors (IGF) in
bovine mammary gland during early involution. Eight mammary quarters from six
nonpregnant cows in late lactation were infused with 10 mL of ginseng extract
solution (3 mg/mL), six quarters were treated with 10 mL of placebo (vehicle
alone) and six quarters were maintained as uninoculated controls. Milking was
interrupted after infusion. Concentrations of IGF1 in mammary secretions were
higher in GS-treated quarters than in placebo and uninoculated control quarters
at 24, 48 and 72 h post-treatment (p < 0.05).
Treatment with GS did not affect mammary secretion of IGF2 (p =
0.942). At 7 d of post-lactational involution, a decrease of immunostained area
and mRNA expression for IGF1 was observed in mammary tissue of GS-treated
quarters compared with placebo-treated quarters and uninoculated controls (p <
0.05). The IGF2 immunostained area and mRNA expression for this growth factor
were not affected by GS treatment (p = 0.216 and p =
0.785, respectively). An increase in protein levels and mRNA expression in
mammary tissue of IGFBP3, IGFBP4 and IGFBP5 was observed in GS-treated quarters
compared with placebo-treated quarters and uninoculated controls (p <
0.05). These results provide evidence that intramammary inoculation of GS
extract at cessation of milking may promote early mammary involution through
the inhibition of IGF1 local production and bioavailability.