Study of genes regulated by the glucocorticoid receptor in peripheral blood and pleural fluid mononuclear cells from patients with pleural tuberculosis

GALLUCCI G; MASSA E; IMHOFF M; BONGIOVANNI B; DIAZ A; SANTUCCI N; BERTOLA D; LIOI S; DERIO M; BAY ML; BOTTASSO O; D´ATTILIO L

Buenos Aires

Congreso; LXVI Reunión Anual de la SAIC, 17-20 noviembre de 2021; 2021

Sociedad Argentina de Investigación Clínica

One of the commonest extrapulmonary manifestations of tuberculosis (TB) is pleural TB (PLTB). Since the cellular immune response (IR) is essential for the containment and resolution of the infectious process, PLTB constitutes a model to study the protective IR at the site of infection, and the ensuing endocrine response. In previous studies, we have shown that patients with PLTB have a greater inflammatory and cellular response at the pleural compartment (increase in IL-1β, IL-6 and IFN-ɣ concentrations) respect to the systemic level. Furthermore, mononuclear cells (MC) from pleural exudates (PEMC) had an in vitro high specific proliferative capacity compared to peripheral counterparts (PBMC), with cortisol levels being only increased at the peripheral compartment. To expand this issue, PBMC and PEMC from PLTB patients (n=12) were analyzed for the expression levels of genes (RT-qPCR) that are positively (ANXA1, GILZ, FKBP5, NFKBIA and NFKBIB) or negatively (IL-1β, IL-6, IFN-ɣ) regulated by glucocorticoid receptor (GR), in addition to the NFkB subunit 1 gene (NFkB1) and the eventual relationship with the circulating immuno-endocrine profile. While showing an increase in the expression levels of ANXA1, GILZ, NFKBIA in PBMCwith respect to PEMC (p