Levels of interleukin-8 in tuberculous pleurisy and the profile ofimmunocompetent cells in pleural and peripheral compartments

DLUGOVITZKY D; RATENI L; TORRES MORALES A; RUIZ SILVIA A; PIÑESKY R; CANOSA B; MOLTENI O; BOTTASSO O; BOTTASSO OSCAR

IMMUNOLOGY LETTERS

ELSEVIER SCIENCE BV

Lugar: Estocolmo; Año: 1997 vol. 55 p. 35 - 40

0165-2478

Our study investigated the presence of IL-8 in pleural exudates from tuberculosis patients (TBP) (II = 1.7). and evaluated whether it was related with the profile of major immunocompetent cells present in their pleural and peripheral compartments. Toallow comparisons, an additional group of patients with parapneumonic pleural effusions (PNE) (II = 7) \vas included. Blood peripheral immunophenotypic studies were also carried out in I2 age-matched healthy controls (Co). and 39 tuberculosis patients classified. according to the extent of pulmonary involvement, into mild (II = 9). and advanced (II = 3)) cases. Patients were recruited before starting therapy, had HIV negative serology. and showed no age differences. IL-8 concentrations were measured by an ELISA method while immunophenotipic analysis was performed by using FITC-conjugated monoclonal antibodies reacting against the following cell surface molecules: CD3. CD-!, C?DX. CD25(IL-2R ? cells), CDl9. and CD68. IL-8 was detected in all pleural exudates though levels in the TB patients. appeared significantly higher than the PNE group, I85 i I IO pg:?mg, (P < 0.015. mean + S.D.). In turn. the former group presented values of pleural CD3 + . CD4 + . and CD25. which were found increased in comparison with PNE patients (P < 0.01 ).Unlike the pleural compartment. patients with TBP showed a marked and significant decrease in their circulating le\rls of cells bearing the CD3. CD4, CDl9. CD25. and CD68 phenotypes not only when comparing with Co but also with PNt: and mildpatients. Differences between the levels of pleural and peripheral T-cells from TBP patients may be the reflection of an important influx of T-lymphocytes from the circulatory system to the pleural cavity. probably linked to the presence of chemolactic factor within the pleural fluid like IL-8.