Ácidos grasos dietarios y marcadores inflamatorios en saliva de pacientes con enfermedades liquenoides bucales y cáncer bucal.

COSTANTINO E; CASTELL SD; PANICO R; PASQUALINI ME; PISTORESI MC; ACTIS AB

Buenos Aires

Congreso; VII Congreso de la Región Latinoamericana de la International Association of Dental Research (IADR). LIV Reunión Anual de la Sociedad Argentina de Investigación Odontológica (SAIO).; 2021

Sociedad Argentina de Investigación Odontológica (SAIO).

Objective: to analyze the relationship between dietary fatty acids (FA) and inflammatory salivary markers in oral lichenoid diseases (OLD) and oral squamous cell carcinoma (OSCC).Patients and methods: 31 persons bearing OLD (n=20) and OSCC (n=11), and 44 healthy volunteers (H) (40-80 years old in all groups) were included. A validated qualitative-quantitative food frequency questionnaire was employed. Data were processed using Interfood v.1.3. Mixed unstimulated saliva was obtained from each participant to analyze the following cytokines and eicosanoids: interleukins 1β (IL-1β), 6 (IL-6), tumor necrosis factor (TNF) (ELISA) and 12-hydroxyheptadecatrienoic acid (12-HHT), 13-hydroxyoctadecadienoic acid (13-HODE), 12 and 5 hydroxyeicosatetraenoic acid (12 and 5-HETE) (HPLC). Kruskal Wallis and Spearman?s coefficient tests were employed (p<0.05). Results: IL-6 and TNF levels were higher in oral lesion patients than in H (p<0.001 and p=0.005, respectively). 13-HODE, 5 and 12-HETE were higher in OLD than in OSCC, and their levels were also higher in diseased than in H persons (p=0.002). The following negative associations were found in H: IL-6 and docosahexaenoic FA (-0.37; p=0.013); TNF and dietary n-3 eicosapentaenoic (-0.52; p<0.001) and docosahexaenoic FA (-0.94; p<0.001). Positive associations were found between IL-6 and arachidic FA (0.73; p=0.01) and IL-1β and lauric saturated FA (0.65; p=0.038) in OSCC, as well as between 12-HHT and n-6 linoleic FA (0.61; p=0.041), and 5-HETE and n-6 arachidonic FA (0.63; p=0.043) in OLD. The following negative associations were found in OSCC: 12-HHT and dietary n-6 gamma-linolenic FA (-0.64; p=0.033); 13-HODE and dietary n-6 linoleic FA (-0.72, p=0.013).Conclusion: the intake of n-3, n-6 and saturated FA appear to influence the inflammatory state of the oral mucosa by modulating the production of different cytokines and eicosanoids. Further research is necessary in order to contribute to oral cancer prevention and early diagnosis.