Resolvinas derivadas de los ácidos grasos n-3 dietarios: influencia sobre el cáncer bucal en ratones

COMBINA HERRERA C; CREMONEZZI DC; PASQUALINI ME; ACTIS AB

Buenos Aires

Congreso; VII Congreso de la Región Latinoamericana de la International Association of Dental Research (IADR). LIV Reunión Científica Anual de la Sociedad Argentina de Investigación Odontológica (SAIO); 2021

Sociedad Argentina de Investigación Odontológica (SAIO)

Objective: To assessthe relationship between resolvins (Rv) derived from n-3 dietary fatty acids(FAs) and carcinogenesis in mouse lingual mucosa. Methods:24 male BALB/c mice were assigned to two dietary groups: chia (ChG;n=12) andcontrol (CG;n=12). Animals in each group were fed on a semi-softdiet with soybean oil (rich in n-6 FA) and chia oil (rich in n-3 FA) (6%),respectively, as a lipid source. Tumors were induced by topical application of4NQO on the tongue dorsum, 3 times a week, for 16 weeks (W). Six animals out ofeach group were sacrificed at 32 and 40 W. Tongues with their tumors and bloodsamples were obtained. Blood was centrifuged to get plasma (P). Tissue (T) sampleswere processed to analyze histopathological changes (HE/light microscopy). RvD1, D2, D3, D5 and E2, derived from n-3 FAs, were analyzed in P and T by highperformance liquid chromatography (HPLC) coupled to mass spectrometry (MS). Valuesare expressed as mass concentration. The Kruskal Wallis test was applied (p<0.05).Results:Oralsquamous cell carcinomas (OSCC) were found in 16.6% of animals in CG while notumor was detected in ChG, both at 32 and 40 W. The following Rv mean values werefound at 32 W: 239,01 (T) and 671,5 (P) in CG;1067,27 (T) and 2243,03 (P) in ChG.At 40 W, Rv levels were 280,05 (T) and 554,33 (P) in CG; 1514,83 (T) and 2949,8 (P) in ChG. Rv were higher in ChG than in CG both at 32 (p=0.004) and 40 W (p=0.002), being higher in P than in T and in 40 W with respect to 32W. Conclusion: Thesepreliminary results show that Rv derived from dietary n-3 FAs modulate thedevelopment and progression of tongue OSCC in mice with a noticeable protectiveeffect.