Dietary lipids change the expression of a proliferation marker in DMBA-induced salivary tumors

ACTIS AB; CREMONEZZI DC; LAMPE PD; CARINO S; VALENTICH MA

JOURNAL OF FOOD LIPIDS

WILEY-BLACKWELL PUBLISHING, INC

Año: 2009 vol. 16 p. 314 - 324

1065-7258

Salivary tumors were induced by 9,10-dimethyl-1,2-benzanthracene injection into the submandibular gland of mice fed with different dietary lipids. Tumor tissue from 10 mice fed with diets containing corn oil (CO) or fish oil (FO) were immunohistochemically stained for Cx26, â- and ã-catenin, BAX, Bcl-2 and Ki-67. The percentage of animals showing different levels of biomarker expression in epithelial, mesenchymal and stromal cells was calculated in each group.  The number of nuclei positive for Ki-67 per 500 total cells was calculated. Reduced expression of Cx26 was observed in FO group as compared to CO. The staining was similar for â-catenin, ã-catenin, BAX and Bcl-2 in both groups. The Ki-67 labeling index was significantly different for CO and FO (0.45-0.40, P<0.0008). The lower expression of Ki-67 in FO mice could indicate less cellular proliferation.