Utilization of Discard Bovine Bone as a Support for Immobilization

ADRIANA CLEMENTZ; GONZALO DEL PESO; ALBERT CANET; JUAN C. YORI; FRANCISCO VALERO

BIOTECHNOLOGY PROGRESS

AMER CHEMICAL SOC

Lugar: Washington; Año: 2016 vol. 32 p. 1243 - 1253

8756-7938

In this study the possibility of using discard bovine bone as support for immobilization ofRhizopus oryzae lipase expressed in Pichia pastoris was analyzed. Discard bovine bone weremilled and then subjected to a chemical treatment with acetone in order to remove lipidsand blood traces. Two types of supports were evaluated: bovine bone and calcined bovinebone for 2 h at 6008C. Supports were characterized by: ICP, SEM, XRD, FTIR, XPS, andN2 adsorption isotherms. Calcined bovine bone presented appropriate characteristics for thelipase immobilization due to the removal of collagen: high porosity, large surface area andsuitable porous structure. Biocatalysts were prepared with different initial enzyme load. Forthe equilibrium adsorption studies, the Langmuir isotherm was used to fit the data results.The immobilization occurs in monolayer to a value of 35 UA mg21. The activities of biocatalystswere tested in transesterification reaction of olive oil. For the enzyme load used inthe test, a final yield percentage of 49.6 was achieved after six methanol additions and 180min of reaction, similar values were obtained using Relizyme as support. Therefore, thebovine bone discard is an economical and appropriate choice for use support immobilizationof enzymes