Determination of Bobel-24 anti-inflammatory and its metabolites in human urine by HPLC-ICP-MS

WUILLOUD, RODOLFO G.; SELAR, NIRANJAN; KANNAMKUMARATH, SASI S.; CARUSO, JOSEPH A.

Fort Lauderdale, Florida

Conferencia; 2004 WINTER CONFERENCE ON PLASMA SPECTROCHEMISTRY; 2004

WINTER CONFERENCE ON PLASMA SPECTROCHEMISTRY

Bobel-24 is a new iodine-containing drug whose anti-inflammatory properties have been tested ??in vivo?? in several animal inflammation models. It has been found that Bobel-24 is a 5-lipoxygenase inhibitor1 which could be effective in the therapy of inflammatory diseases mediated by leukotrienes. Currently, studies are being developed to evaluate whether Bobel-24 is effective in the treatment of articular diseases and seborrhoeic dermatitis. The success of these studies, depend on rapid and simple analytical methodologies, available for the proper determination of Bobel-24 in different biological samples. A method using high-performance liquid chromatography (HPLC) with UV absorbance detection for pharmacokinetic studies of Bobel-24 has been described in the past.1 However, the high detection limits in the mg l-1 range may prohibit the determination of Bobel-24 and its metabolites in biological samples. The use of inductively coupled plasma mass spectrometry (ICP-MS) as a detector could fulfill the sensitivity requirements for the determination of Bobel-24 (2,4,6-triiodophenol) in biological samples at low concentrations.In this work, HPLC was coupled on-line to ICP-MS for separation and determination of Bobel-24 anti-inflammatory and its metabolites in human urine. Separation was obtained using anion-exchange chromatography in aqueous phase eliminating the possibility of sensitivity deterioration due to organic solvent introduction into the ICP-MS detector. Detection limits in the low ug l-1 range were obtained or all the iodinated species. Different extraction procedures of the iodinated compounds from the urine samples were also evaluated.