Speciation of essential and toxic elements in edible mushrooms: size exclusion chromatography with on-line UV-inductively coupled plasma mass spectrometry detection

WUILLOUD, RODOLFO G.; KANNAMKUMARATH, SASI S.; CARUSO, JOSEPH A.

APPLIED ORGANOMETALLIC CHEMISTRY

WILEY & SONS

Año: 2004 vol. 18 p. 156 - 165

0268-2605

Size-exclusion liquid chromatography was coupled to UV and inductively coupled plasma mass spectrometry (ICP-MS) for detection to perform elemental speciation studies on different edible mushrooms. Molecular weight (MW) distribution patterns of several elements among different fractions present in various edible mushrooms are presented. The association of the elements with the high and low MW fractions was observed using sequential detection by UV and ICP-MS. Separation was performed using a Superdex 75 column. Variability of the fractionation patterns with three different extraction media (0.05 mol 1-1 NaOH; 0.05 mol 1-1 HCl; hot water at 60 °C) was evaluated for mushroom species. A comparative elemental speciation study was performed in order to determine the differences in the fractionation patterns of silver, arsenic, cadmium, mercury, lead, and tin in Boletus edulis, Agaricus bisporus, and Lentinus edodes. Differences in the fractionation patterns of the elements were found to depend on the mushroom species and the extraction medium. Most of the elements were associated with high mw fractions. It was not possible to assess the trace metal contributions from the mushroom growth media.