Determination of cobalt in urine by FI-ICP-AES with on-line preconcentration

FARIAS, GUSTAVO F.; WUILLOUD, RODOLFO G.; MOYANO, SUSANA; GÁSQUEZ, JOSÉ A.; OLSINA, ROBERTO A.; MARTINEZ, LUIS D.

JOURNAL OF ANALYTICAL TOXICOLOGY

S. Tinsley Preston

Año: 2002 vol. 26 p. 360 - 364

0146-4760

A method for the preconcentration and determination of cobalt in human urine samples was developed. The online preconcentration and determination were attained using inductively coupled plasma atomic emission spectromety (ICP-AES) coupled to a flow injection (FI) method. Cobalt was retained on an Amberlite XAD-7 resin as cobalt-2-(5-bromo-2-pyridylazo)-5-diethylaminophenol complex at pH 9.5. Cobalt was removed from the microcolumn with perchloric acid. A sensitivity enhancement factor of 90 was obtained with respect to cobalt determination by ICP-AES without preconcentration. The value of detection limit for the preconcentration method proposed was 25 ng/L. The precision for 10 replicate determinations at the 5 mg/L (mean ± SD, 5.1 ± 0.14) Co level was 2.7% relative standard deviation, calculated from the peak heights obtained. The calibration graph preconcentration method for cobalt was linear with a correlation coefficient of 0.9994 from approximately 0.25 mg/L up to at least 100 mg/L. The method was successfully applied to the determination of cobalt in human urine samples.