IGF-1 and growth hormone receptor type 2 (GHR2) in Cichlasoma dimerus: sequencing, tissue specific expression and regulation by fasting

TOMÁS DELGADIN; PÉREZ SIRKIN DANIELA IRINA; ARRANZ,SE; VISSIO, PAULA G.

Buenos Aires

Congreso; 7th INTERNATIONAL SYMPOSIUM ON FISH ENDOCRINOLOGY (7ISFE); 2012

International Commitee

Teleosts are known to be a diverse group of fish, some of which are capable of enduring long periods of food restriction. The aim of the present study was to investigate the effect of fasting on somatic growth, liver histology and IGF-1 and GHR2 expression in C. dimerus. GHR2 and IGF-1 transcripts were sequenced from liver, and their tissue distribution and regulation by fasting were studied by RT-PCR and RTqPCR respectively. Both IGF-1 and GHR2 transcripts showed a widespread distribution, been detected in pituitary, whole brain, liver and gonads among others. Fasting for 4 weeks resulted in a significant reduction in body weight, total and standard length, as well as in hepatosomatic index and hepatocyte area (p